Jang Min-Ho, Ha Kyong, Takamura Noriko
Department of Biology Education, Kongju National University, Kongju, 314-701, South Korea.
Toxicon. 2008 Apr;51(5):882-9. doi: 10.1016/j.toxicon.2007.12.015. Epub 2007 Dec 23.
Microcystin (MC) production by four monoclonal Microcystis aeruginosa strains was evaluated in response to infochemicals (indirect exposure) released from different stages of herbivorous zooplankton (neonate/juvenile and adult Daphnia magna and Moina macrocopa). The intracellular MC and extracellular MC concentrations were significantly different among the control and treatments with zooplankton culture media filtrates (p<0.05), and in most cases MC production was significantly higher (p<0.05) in strains exposed to infochemicals released from adult zooplankton rather than those of neonate/juvenile zooplankton in four strains of M. aeruginosa. Compared to intracellular MC (385.0-5598.6microg g(-1)DW), very low concentrations of extracellular MC (9.9-737.6microg ml(-1)) were released, but both showed similar temporal patterns over the course of the experiment. This result might be attributed to the fact that adult zooplankton produced more infochemical signals than equal numbers of smaller juveniles and neonates. It is the first study to provide evidence that MC production might be impacted by infochemicals released from different stages of zooplankton, mediated with physiological characteristics, body size, and feeding habits.
研究评估了四种铜绿微囊藻单克隆菌株对食草性浮游动物（大型溞和多刺裸腹溞的幼体/幼龄个体和成体）不同发育阶段释放的信息化学物质（间接暴露）的微囊藻毒素（MC）产生情况。对照和添加浮游动物培养基滤液的处理组之间，细胞内MC和细胞外MC浓度存在显著差异（p<0.05），并且在大多数情况下，在暴露于成年浮游动物释放的信息化学物质的铜绿微囊藻菌株中，MC产量显著更高（p<0.05），高于暴露于幼体/幼龄浮游动物释放的信息化学物质的菌株。与细胞内MC（385.0 - 5598.6μg g(-1)干重）相比，释放的细胞外MC浓度非常低（9.9 - 737.6μg ml(-1)），但在实验过程中两者呈现出相似的时间模式。这一结果可能归因于成年浮游动物比等量的较小幼体和幼龄个体产生更多信息化学信号这一事实。这是第一项提供证据表明MC产生可能受到浮游动物不同发育阶段释放的信息化学物质影响的研究，这种影响由生理特征、体型和摄食习惯介导。
