Snider Rachel M, Ciobanu Madalina, Rue Amy E, Cliffel David E
Department of Chemistry, Vanderbilt University, Nashville, TN 37235-1822, United States.
Anal Chim Acta. 2008 Feb 18;609(1):44-52. doi: 10.1016/j.aca.2007.12.032. Epub 2008 Jan 6.
We have developed a multiwalled carbon nanotube/dihydropyran (MWCNT/DHP) composite sensor for the electrochemical detection of insulin in a microfluidic device. This sensor has been employed for physiological measurements of secreted insulin from pancreatic islets in a Cytosensor previously modified to be a multianalyte microphysiometer (MAMP). When compared with other established electrochemical insulin sensors, the MWCNT/DHP composite film sensor presented improved resistance to fluidic shear forces, while achieving enhanced electrode kinetics. In addition, the preparation of the composite film is straightforward and facile with a self-polymerizing monomer, DHP, used to add mechanical stability to the film. The sensor film was able to detect insulin concentrations as low as 1muM in the MAMP during calibration experiments. The MWCNT/DHP composite sensor has been successfully used for the direct detection of insulin secreted by islets in the microphysiometer.
我们开发了一种用于在微流控装置中对胰岛素进行电化学检测的多壁碳纳米管/二氢吡喃(MWCNT/DHP)复合传感器。该传感器已用于在先前改装为多分析物微生理计(MAMP)的细胞传感器中对胰岛分泌的胰岛素进行生理测量。与其他已有的电化学胰岛素传感器相比,MWCNT/DHP复合膜传感器对流体剪切力具有更好的抗性,同时实现了增强的电极动力学。此外,复合膜的制备简单易行,使用自聚合单体DHP来增加膜的机械稳定性。在校准实验中,传感器膜能够在MAMP中检测低至1μM的胰岛素浓度。MWCNT/DHP复合传感器已成功用于微生理计中对胰岛分泌的胰岛素进行直接检测。