Zhan Zhikun, Zhang Hongyu, Niu Xuanyu, Yu Xiaodong, Sun Hui, Sha Xiaopeng, Zhao Yuliang, Wang Ying, Li Wen Jung
Key Laboratory of Intelligent Rehabilitation and Neromodulation of Hebei Province, Yanshan University at Qinhuangdao, Qinhuangdao 066004, China.
School of Control Engineering, Northeastern University at Qinhuangdao, Qinhuangdao 066004, China.
ACS Omega. 2020 Mar 10;5(11):6169-6176. doi: 10.1021/acsomega.0c00194. eCollection 2020 Mar 24.
The monitoring of insulin, which is the only hormone that helps regulate blood glucose levels in the body, plays a key role in the diagnosis and treatment of diabetes. However, most techniques today involve complicated electrode fabrication and testing processes, which are time-consuming and costly, and require a relatively large volume of sample. To overcome these drawbacks, we present here a low-cost insulin detection method based on a screen-printed electrode (SPE) modified by nickel hydroxide (Ni(OH)). This novel method only requires 300 μL of insulin sample, and the time it takes for electrode preparation is about 12 times shorter than traditional electrode fabrication methods such as coating and sol-gel methods. The electrochemical behaviors of the Ni(OH)-coated SPE (NSPE) sensing area in insulin aqueous solutions are studied using cyclic voltammetry, amperometric - curves, and electrochemical impedance spectroscopy. The results demonstrate that the NSPE sensing surface has excellent detection properties, such as a high sensitivity of 15.3 μA·μM and a low detection limit of 138 nM. It takes a short time (∼10 min) to prepare the NSPE sensing surface, and only two drops (∼300 μL) of insulin samples are required in the detection process. Moreover, the selectivity of this method for insulin detection is verified by detecting mixtures of insulin and ascorbic acid or bovine hemoglobin. Finally, we discuss the potential clinical applications of this method by detecting various concentrations of insulin in human serum.
胰岛素是体内唯一有助于调节血糖水平的激素，对其进行监测在糖尿病的诊断和治疗中起着关键作用。然而，当今大多数技术都涉及复杂的电极制造和测试过程，这些过程既耗时又昂贵，而且需要相对大量的样本。为克服这些缺点，我们在此提出一种基于用氢氧化镍（Ni(OH)）修饰的丝网印刷电极（SPE）的低成本胰岛素检测方法。这种新方法仅需300μL胰岛素样本，且电极制备所需时间比诸如涂层法和溶胶 - 凝胶法等传统电极制造方法短约12倍。使用循环伏安法、安培曲线和电化学阻抗谱研究了涂覆Ni(OH)的SPE（NSPE）传感区域在胰岛素水溶液中的电化学行为。结果表明，NSPE传感表面具有优异的检测性能，如15.3μA·μM的高灵敏度和138 nM的低检测限。制备NSPE传感表面耗时短（约10分钟），检测过程中仅需两滴（约300μL）胰岛素样本。此外，通过检测胰岛素与抗坏血酸或牛血红蛋白的混合物验证了该方法对胰岛素检测的选择性。最后，我们通过检测人血清中不同浓度的胰岛素来讨论该方法的潜在临床应用。
