[HLA-A, B, and DRB1 gene polymorphisms in Mongol ethnic group of Inner Mongolia, China].

We investigated the genetic polymorphisms of HLA-A, B, DRB1 alleles and haplotype frequencies in Mongols from Inner Mongolia, China. A PCR-based reverse line-strip sequence specific oligonucleotide hybridization method was used to determine the alleles of HLA-A, B, DRB1 in 106 unrelated, healthy Mongol individuals of Inner Mongolia. Allelic and haplotype frequencies of these loci were calculated by the maximum likelihood estimator method. Altogether, 13 HLA-A alleles, 29 HLA-B alleles and 13 HLA-DRB1 alleles were observed in the population. The most frequent HLA A-B, B-DRB1, and A-B-DRB1 haplotypes were HLA-A02-B46 (0.0510), HLA-A02-B13 (0.0495), HLA-A02-B51 (0.0442); HLA-B13-DRB107(0.0555), HLA B46-DRB109 (0.0378), HLA-B35-DRB113(0.033); and HLA-A02-B13- DRB107(0.033019), HLA-A02-B46-DRB109 (0.031985), respectively. These results indicated that the HLA loci are highly polymorphic in Mongols of Inner Mongolia, and haplotypes HLA-A24-B14, HLA-A32-B63 have significant linkage disequilibrium.