[采用液相色谱-串联质谱法测定动物肾脏和尿液中氯丙嗪残留量]

[Determination of chloropromazine residues in animals kidney and urine using LC-MS/MS method].

作者信息

Rodziewicz Lech, Zawadzka Iwona

机构信息

Pracownia Badań Chemicznych Srodków Spozywczych, Zakład Higieny Weterynaryjnej 15-959 Białystok, ul. Zwyciestwa 26a.

出版信息

Rocz Panstw Zakl Hig. 2007;58(3):503-8.

PMID:18246654

Abstract

Chloropromazina (CP) is subjected to monitoring food animals products, with a minimum required performance limit (MPRL) set 5.0 microg/kg. Homogenized kidney and urine were extracted with acetonitrile. CP- d3 was used as internal standard. LC separation was done on Luna C18 150 x 2 mm, 5 microm column in mobile phase acetonitrile-acetic acid. CP was determination by LC-ESI-MS/MS negative mode. The method was validation according to the criteria of Decision Commission No 2002/657/EC. Recoveries for the level 5.0 ng/g were in the range 84-102%. The limit of decision (CCalpha) and detection capability (CCbeta) CP in kidney were 1.19; 2.87 ng/g and urine 1.08; 2.61 ng/g.

摘要

氯丙嗪（CP）被用于监测食用动物产品，设定的最低要求性能限量（MPRL）为5.0微克/千克。用乙腈提取匀浆后的肾脏和尿液。CP-d3用作内标。在Luna C18 150×2毫米、5微米的色谱柱上，以乙腈 - 乙酸为流动相进行液相色谱分离。通过液相色谱 - 电喷雾串联质谱（LC-ESI-MS/MS）负模式测定CP。该方法根据欧盟委员会第2002/657/EC号决定的标准进行了验证。5.0纳克/克水平的回收率在84 - 102%范围内。肾脏中CP的决策限（CCα）和检测能力（CCβ）分别为1.19；2.87纳克/克，尿液中为1.08；2.61纳克/克。