淋巴瘤细胞U937增殖过程中p27(kip1)及其相关分子Jab1和CRM1的表达及关系
[Expression and relationship of p27(kip1) and its related molecules Jab1 and CRM1 during proliferation of lymphoma cells U937].
作者信息
Wang Yu-Chan, Zhang Dong-Mei, Shen Ai-Guo, Lu Jian-Xing, Shao Xiao-Yi, He Song, Cheng Chun
机构信息
Department of Microbiology and Immunology, Nantong University, Nantong 226001, China.
出版信息
Zhonghua Zhong Liu Za Zhi. 2007 Sep;29(9):657-61.
OBJECTIVE
To investigate the expression and relationship of p27(kip1) and its related molecules Jab1 and CRM1 during proliferation of lymphoma cells U937.
METHODS
U937 cells were treated with serum starvation and release, and the effects of these treatments on the cell growth was tested with cell number counting. The expression and localization of p27(kip1), Jab1 and CRM1 in U937 cells were detected by Western blot, double immunolabelling and laser scanning confocal microscopy.
RESULTS
The growth of U937 cells was blocked by serum starvation. The total protein of p27(kip1) was increased while Ser10-phosphorylated p27(kip1) -related molecules Jab1 and CRM1 were decreased. Meanwhile, the location of p27(kip1) was changed from cytoplasm into nuclei. After serum release, the location of p27(kip1) expression reappeared in the cytoplasm again.
CONCLUSION
During the proliferation process of lymphoma U937 cells, Jab1 and CRM1 may influence the location and expression of p27kip1, and may participate in regulation of growth of NHL cells.
目的
研究淋巴瘤细胞U937增殖过程中p27(kip1)及其相关分子Jab1和CRM1的表达及关系。
方法
对U937细胞进行血清饥饿及再释放处理，通过细胞计数检测这些处理对细胞生长的影响。采用蛋白质免疫印迹法、双重免疫标记法及激光扫描共聚焦显微镜检测U937细胞中p27(kip1)、Jab1和CRM1的表达及定位。
结果
血清饥饿可阻断U937细胞生长。p27(kip1)总蛋白增加，而Ser10磷酸化的p27(kip1)相关分子Jab1和CRM1减少。同时，p27(kip1)的定位从细胞质转移至细胞核。血清再释放后，p27(kip1)表达定位又重新出现在细胞质中。
结论
在淋巴瘤U937细胞增殖过程中，Jab1和CRM1可能影响p27kip1的定位和表达，并可能参与非霍奇金淋巴瘤细胞生长的调控。
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