Rodini Camila Oliveira, Batista Aline Carvalho, Dionísio Thiago José, Santos Carlos Ferreira, Cunha Fernando Queiroz, Lara Vanessa Soares
Department of Stomatology Oral Pathology, Bauru School of Dentistry, University of São Paulo, Sao Paulo, Brazil.
J Mol Histol. 2008 Jun;39(3):275-82. doi: 10.1007/s10735-008-9163-4. Epub 2008 Feb 5.
The immunopathologic and inflammatory mechanisms involved in periodontal disease (PD) include the participation of host resident, inflammatory cells and chemical mediators. Metalloproteinases (MMPs) and nitric oxide (NO) play essential role in extracellular matrix turnover of periodontal tissue destruction. In this study, by means of RT-PCR through semi-quantitative densitometric scanning methods, the expression of MMPs -2 and -9 and inducible NO synthase (iNOS) was temporally and spatially investigated during the destructive mechanisms of experimentally induced PD in rats. Samples from different periods were microscopically analyzed and compared with the contralateral side (control). Our results showed significant expression of MMP-9 and iNOS in tissues affected by PD, as compared with controls, three days after PD induction, simultaneously with the beginning of alveolar bone loss. At 7 days post induction, only the MMP-9 mRNA presented a significantly higher expression, as compared with the respective controls. Thus, in the rat ligature-induced PD, MMP-9 and iNOS might importantly participate in the early stages of the disease, including inflammatory cell migration, tissue destruction and alveolar bone resorption. Also, we may suggest that the exuberant presence of PMNs may be related to the important expression of iNOS and MMP-9 found at 3 days post induction.
牙周病（PD）所涉及的免疫病理和炎症机制包括宿主固有细胞、炎症细胞和化学介质的参与。金属蛋白酶（MMPs）和一氧化氮（NO）在牙周组织破坏的细胞外基质周转中起重要作用。在本研究中，通过逆转录聚合酶链反应（RT-PCR）和半定量密度扫描方法，在实验诱导大鼠牙周病的破坏机制过程中，对MMPs -2和 -9以及诱导型一氧化氮合酶（iNOS）的表达进行了时空研究。对不同时期的样本进行显微镜分析，并与对侧（对照）进行比较。我们的结果显示，与对照组相比，在诱导牙周病三天后，即牙槽骨丧失开始时，受牙周病影响的组织中MMP-9和iNOS有显著表达。诱导后7天，与各自的对照组相比，只有MMP-9 mRNA呈现出显著更高的表达。因此，在大鼠结扎诱导的牙周病中，MMP-9和iNOS可能在疾病的早期阶段重要地参与其中，包括炎症细胞迁移、组织破坏和牙槽骨吸收。此外，我们可以推测，中性粒细胞的大量存在可能与诱导后3天发现的iNOS和MMP-9的重要表达有关。
