Suzuki S, Oka H, Yasuda H, Yamashita K, Kaneko T, Oda T
Endocrinol Jpn. 1975 Dec;22(6):479-82. doi: 10.1507/endocrj1954.22.479.
Time course of the changes in insulin release and cyclic AMP levels in isolated rat islets incubated in media containing 5 or 16.7 mM of glucose were followed. The higher glucose concentration caused a slight but significant increase of cyclic AMP levels after 10 min incubation, but not 5 min incubation, whereas the stimulation of insulin release by 16.7 mM of glucose was apparent in both incubation times. Theophylline increased cyclic AMP levels markedly but did not stimulate insulin release when the glucose concentration was 5 mM. A slight augmentation by theophylline of insulin release was observed in the incubation medium containing 16.7 mM glucose. All these findings suggest that the elevation of cyclic AMP in islets may not play a role for the initiation of the insulin release induced by glucose, though it may act to modulate the glucose effect.
对在含有5或16.7 mM葡萄糖的培养基中孵育的分离大鼠胰岛中胰岛素释放和环磷酸腺苷（cAMP）水平变化的时间进程进行了跟踪。较高的葡萄糖浓度在孵育10分钟后导致cAMP水平轻微但显著升高，但在孵育5分钟时未出现这种情况，而16.7 mM葡萄糖对胰岛素释放的刺激在两个孵育时间均很明显。当葡萄糖浓度为5 mM时，茶碱显著提高了cAMP水平，但未刺激胰岛素释放。在含有16.7 mM葡萄糖的孵育培养基中观察到茶碱对胰岛素释放有轻微增强作用。所有这些发现表明，胰岛中cAMP的升高可能在葡萄糖诱导的胰岛素释放起始过程中不起作用，尽管它可能起到调节葡萄糖效应的作用。
