Tojo S, Ashitaka Y, Maruo T, Nishimoto H
Endocrinol Jpn. 1975 Dec;22(6):585-9. doi: 10.1507/endocrj1954.22.585.
The biological properties of human chorionic FSH (hCFSH) for rat ovaries were investigated. Highly purified hCFSH had similar response to the ovarian augmentation test as bovine FSH and significantly enhanced 3H-thymidine uptake by granulosa cells and theca cells in the ovary of hypophysectomized rat. In contrast, highly purified hCG little responded to the ovarian augmentation test and had no effect on 3H-thymidine uptake by the ovary. These results indicate that hCFSH may promote the follicular growth of ovary resulting from granulosa cell proliferation and its enlargement. In addition, freshly harvested porcine granulosa cells were employed in an in vitro system to investigate specific binding of hCFSH to ovarian receptor. Radioiodinated hCFSH (125I-hCFSH) and hCG (125I-hCG) were respectively incubated with cell suspensions. Binding of these hormone preparations was proportional to the cell number and increased with the time of incubation through 120 minutes. The binding ability of 125I-hCFSH to the cells was greater than that of 125I-hCG. Increasing concentrations of unlabeled hCFSH in the incubation mixture progressively inhibited the uptake of 125I-hCFSH by granulosa cells. Unlabeled hCG was not able to compete with 125I-HCFSH binding. The similar phenomenon to inhibit the binding of 125I-hCG to the cells was also recognized in the presence of unlabeled hCG. These findings suggest that granulosa cell has at least two different types of receptor sites: one for hCFSH and the other for hCG.
研究了人绒毛膜促卵泡素（hCFSH）对大鼠卵巢的生物学特性。高度纯化的hCFSH在卵巢增重试验中的反应与牛促卵泡素相似，并显著增强了垂体切除大鼠卵巢中颗粒细胞和卵泡膜细胞对3H-胸腺嘧啶核苷的摄取。相比之下，高度纯化的hCG对卵巢增重试验几乎没有反应，对卵巢摄取3H-胸腺嘧啶核苷也没有影响。这些结果表明，hCFSH可能通过促进颗粒细胞增殖及其增大来促进卵巢卵泡生长。此外，采用新鲜收获的猪颗粒细胞建立体外系统，研究hCFSH与卵巢受体的特异性结合。将放射性碘化的hCFSH（125I-hCFSH）和hCG（125I-hCG）分别与细胞悬液孵育。这些激素制剂的结合与细胞数量成正比，并在120分钟的孵育时间内随时间增加。125I-hCFSH与细胞的结合能力大于125I-hCG。孵育混合物中未标记hCFSH浓度的增加逐渐抑制颗粒细胞对125I-hCFSH的摄取。未标记的hCG不能与125I-HCFSH结合竞争。在未标记hCG存在的情况下，也观察到了抑制125I-hCG与细胞结合的类似现象。这些发现表明，颗粒细胞至少有两种不同类型的受体位点：一种用于hCFSH，另一种用于hCG。
