Peress N S, Perillo E
Research & Development Service, Department of Veterans Affairs Medical Center, Northport, New York.
Invest Ophthalmol Vis Sci. 1991 Jan;32(1):78-87.
The current ultrastructural study of cultured explants of bovine and rabbit posterior ciliary processes using immunoglobulin (Ig) G-opsonized red blood cells (E-IgG) showed specific IgG Fc receptor-mediated attachment, ingestion, and digestion of IgG-coated erythrocytes by the pigmented epithelium (PE). Nonpigmented epithelial cells (NPE) in the cultured explants and a transformed cell line of bovine NPE, with and without lymphokine stimulation, did not have IgG-receptor activity. The interaction between PE and E-IgG involved the extension of micropseudopods toward adherent E-IgG, the formation of a linear uniform cap of roughly 200 A between opposing cell membranes, the ingestion of E-IgG by PE into a membrane-lined compartment, and the disintegration of the ingested ligand into membranous debris. Disintegration of some surface-associated E-IgG was also observed and was consistent with the release of a lytic substance by the receptor-activated PE.
目前使用免疫球蛋白（Ig）G调理的红细胞（E-IgG）对牛和兔睫状体后突培养外植体进行的超微结构研究显示，色素上皮（PE）通过特异性IgG Fc受体介导对IgG包被的红细胞进行附着、摄取和消化。培养外植体中的无色素上皮细胞（NPE）以及牛NPE的转化细胞系，无论有无淋巴因子刺激，均不具有IgG受体活性。PE与E-IgG之间的相互作用包括向附着的E-IgG延伸微伪足，在相对细胞膜之间形成约200埃的线性均匀帽，PE将E-IgG摄入膜内衬隔室，以及摄入的配体分解为膜性碎片。还观察到一些与表面相关的E-IgG的分解，这与受体激活的PE释放溶细胞物质一致。
