De Oliveira Rodrigo Franco, Oliveira Deise A A Pires, Monteiro Wagner, Zangaro Renato Amaro, Magini Márcio, Soares Cristina Pacheco
Laboratório de Dinâmica de Compartimento Celular, Instituto de Pesquisa e Desenvolvimento (IP&D), UNIVAP, São José dos Campos, São Paulo, Brazil.
Photomed Laser Surg. 2008 Feb;26(1):6-9. doi: 10.1089/pho.2007.2112.
The objective of this study was to compare the effect of low-level laser therapy (LLLT) and low-intensity pulsed ultrasound (LIPUS) on fibroblast cell culture. Several methods, including ultrasound treatment and LLLT, are being used to facilitate tissue repair and healing processes.
L929 fibroblast cell cultures were irradiated with low-level laser energy and LIPUS. Cultures irradiated with ultrasound were divided into five groups: group 1: control (did not receive irradiation); group 2: 0.2 W/cm(2) in pulsed mode at 10% (1:9 duty cycle); group 3: 0.6 W/cm(2) in pulsed mode at 10% (1:9 duty cycle); group 4: 0.2 W/cm(2) in pulsed mode at 20% (2:8 duty cycle); and group 5: 0.6 W/cm(2) in pulsed mode at 20% (2:8 duty cycle). Cultures irradiated with laser energy were divided into three groups: group 1: control (did not receive irradiation); group 2: 6 J/cm(2); and group 3: 50 mJ/cm(2). Each group was irradiated at 24-h intervals, with the following incubation periods post-irradiation: 24, 48, and 72 h; after each irradiation cycle the cultures were analyzed using MTT [3-(4.5-dimethylthiazol-2-yl)-2.5 diphenyltetrazolium bromide].
Analysis of results after LLLT and LIPUS demonstrated that the effect of laser therapy on fibroblast cell culture was greater than that of LIPUS (p < 0.05).
Results demonstrated that LLLT significantly increased fibroblastic activity more than LIPUS. Therefore, in the first and second phases of tissue repair, laser treatment may be more effective than ultrasound treatment.
本研究的目的是比较低强度激光疗法（LLLT）和低强度脉冲超声（LIPUS）对成纤维细胞培养的影响。包括超声治疗和LLLT在内的几种方法正被用于促进组织修复和愈合过程。
用低强度激光能量和LIPUS照射L929成纤维细胞培养物。接受超声照射的培养物分为五组：第1组：对照组（未接受照射）；第2组：脉冲模式下0.2W/cm²，占空比10%（1:9）；第3组：脉冲模式下0.6W/cm²，占空比10%（1:9）；第4组：脉冲模式下0.2W/cm²，占空比20%（2:8）；第5组：脉冲模式下0.6W/cm²，占空比20%（2:8）。接受激光能量照射的培养物分为三组：第1组：对照组（未接受照射）；第2组：6J/cm²；第3组：50mJ/cm²。每组每隔24小时照射一次，照射后培养期如下：24、48和72小时；每次照射周期后，使用MTT[3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐]分析培养物。
LLLT和LIPUS后的结果分析表明激光疗法对成纤维细胞培养的影响大于LIPUS（p<0.05）。
结果表明LLLT比LIPUS更显著地增加了成纤维细胞活性。因此，在组织修复的第一和第二阶段，激光治疗可能比超声治疗更有效。
