Liao Qiu-Lin, Chen Xiao-Dong, Zhao Liang, Ding Yan-Qing
Department of Pathology, Southern Medical University/Guangdong Provincial Key Laboratory of Molecular Tumor Pathology, Guangzhou 510515, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2008 Feb;28(2):154-8.
To screen the tumor specific antigens of nasopharyngeal carcinoma (NPC) in the serum of the patients.
Two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was employed to screen the specific biomarkers of NPC in the sera from 42 healthy volunteers, 37 NPC patients with lymph node metastasis and 27 NPC patients without lymphatic metastasis.
After pretreatment including albumin and immunoglobulin (IgG) depletion and desalting, the sera were subjected to 2-DE and image analysis. The differentially expressed protein spots were identified by MALDI-TOF-MS. Sera pretreatment resulted in better repeatability and resolution on the reference gel because of albumin and IgG depletion. From optimized 2-DE gel images, 29 spots indicating differential protein expression were identified by MALDI-TOF-MS to represent 23 proteins. Transferrin, ZNF544 protein, transthyretin, FAD-synthetase and NM23-H1 proteins were down-regulated and 12-lipoxygenase, serum amyloid A1 protein precursor, cytochrome P450, sICAM-1, cathepsin G and lysine-specific histone demethylase 1 were upregulated in the two groups of NPC patients as compared with the healthy group. Increased expressions of 12-lipoxygenase, sICAM-1, cathepsin G and lysine-specific histone demethylase 1 were detected in NPC patients with lymph node metastasis as compared with the patients without lymph node metastasis, but heat shock protein 70 was expressed only in NPC patients with lymph node metastasis.
The 2-DE-based serum proteome analysis can be useful in detecting the protein expression alterations due to carcinogenesis and development of NPC, and the newly discovered biomarkers might help in the diagnosis of NPC.
筛选鼻咽癌（NPC）患者血清中的肿瘤特异性抗原。
采用二维电泳（2-DE）和基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF-MS）技术，对42名健康志愿者、37名有淋巴结转移的NPC患者和27名无淋巴结转移的NPC患者的血清进行检测，以筛选NPC的特异性生物标志物。
经过去除白蛋白和免疫球蛋白（IgG）以及脱盐等预处理后，对血清进行2-DE和图像分析。通过MALDI-TOF-MS鉴定差异表达的蛋白质斑点。由于去除了白蛋白和IgG，血清预处理使得参考胶上的重复性和分辨率更好。从优化后的2-DE凝胶图像中，通过MALDI-TOF-MS鉴定出29个差异表达的斑点，代表23种蛋白质。与健康组相比，两组NPC患者血清中的转铁蛋白、ZNF544蛋白、甲状腺素转运蛋白、FAD合成酶和NM23-H1蛋白表达下调，而12-脂氧合酶、血清淀粉样蛋白A1蛋白前体、细胞色素P450、可溶性细胞间黏附分子-1（sICAM-1）、组织蛋白酶G和赖氨酸特异性组蛋白去甲基化酶1表达上调。与无淋巴结转移的NPC患者相比，有淋巴结转移的NPC患者血清中12-脂氧合酶、sICAM-1、组织蛋白酶G和赖氨酸特异性组蛋白去甲基化酶1的表达增加，但热休克蛋白70仅在有淋巴结转移的NPC患者中表达。
基于2-DE的血清蛋白质组分析有助于检测NPC发生发展过程中蛋白质表达的变化，新发现的生物标志物可能有助于NPC的诊断。
