Li Li, Xing Fu-qi, Chen Shi-ling, Sun Ling, Li Hong
Department of Obstetrics and Gynecology, Kunming General Hospital of Chengdu Command, Kunming 650032, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2008 Feb;28(2):275-8.
To study the differentially expressed proteins between mature and antral follicles and identify the proteins crucial for follicle development and oocyte fertilization.
Mature follicular fluids were obtained from 48 women after oocyte collection during in vitro fertilization (IVF), and antral follicular fluids were collected from 21 women by follicular puncture. The proteins in the follicular fluids were detected by surface-enhanced laser desorption/ionization-time of flight-mass spectrometry (SELDI-TOF-MS) and weak cation-exchange protein chip (WCX-2). The data were read with PBSII-C type protein chip reader and analyzed with Biomarker Wizard and Biomarker Patterns Software of Ciphergen Company.
In comparison with those in the antral follicular fluid, the concentration of 4 proteins were significantly different in mature follicular fluid, including two up-regulated and two down-regulated proteins.
Significant variation occurs in the proteomics of mature follicular fluid, and the differentially expressed proteins may have close relation to follicle development and oocyte fertilization.
研究成熟卵泡和窦卵泡之间差异表达的蛋白质，并鉴定对卵泡发育和卵母细胞受精至关重要的蛋白质。
从48名接受体外受精（IVF）取卵后的妇女中获取成熟卵泡液，通过卵泡穿刺从21名妇女中收集窦卵泡液。采用表面增强激光解吸/电离飞行时间质谱（SELDI-TOF-MS）和弱阳离子交换蛋白芯片（WCX-2）检测卵泡液中的蛋白质。数据用PBSII-C型蛋白质芯片阅读器读取，并使用Ciphergen公司的Biomarker Wizard和Biomarker Patterns软件进行分析。
与窦卵泡液相比，成熟卵泡液中4种蛋白质的浓度有显著差异，其中2种上调，2种下调。
成熟卵泡液蛋白质组发生显著变化，差异表达的蛋白质可能与卵泡发育和卵母细胞受精密切相关。
Zotero 插件