[运用表面增强激光解吸电离技术分析马尔尼菲青霉菌酵母型与丝状型蛋白质组表达差异]
[Analysis of the difference in proteome expression between yeast form and mould form of Penicillium marneffei using SELDI technique].
作者信息
Liu Dong-Hua, Liu Xiao-Jun, Tan Sheng-Shun
机构信息
Derparment of Dermatology, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China.
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2007 Jan;27(1):59-61.
OBJECTIVE
To compare the difference in protome expression between yeast form and mould form of Penicillium marneffei.
METHODS
Surface enhanced laser desorption/ionization (SELDI) time-of-flight mass spectra were performed to compare the expressed proteins between yeast form and mould form of Penicillium marneffei. Protein profiling was read by PBSIIC ProteinChip Reader and the proteome database was analyzed by Proteinchip Software 3.2.0.
RESULTS
Seventy-five distinct proteins were found in the yeast form and mould form of Penicillium marneffei, in which 10 proteins were up-regulated in yeast form and 3 in mould form. The proteins 2900 and 3151 were only expressed in the yeast form and the proteins 13,151 and 13,285 only in mould form.
CONCLUSION
SELDI technique can identify the difference of the expressed low-molecular-mass proteins between the mould form and yeast form of Penicillium marneffei.
目的
比较马尔尼菲青霉菌酵母型和丝状型之间蛋白质组表达的差异。
方法
采用表面增强激光解吸/电离（SELDI）飞行时间质谱法比较马尔尼菲青霉菌酵母型和丝状型之间的表达蛋白。通过PBSIIC蛋白质芯片阅读器读取蛋白质谱，并使用Proteinchip Software 3.2.0分析蛋白质组数据库。
结果
在马尔尼菲青霉菌酵母型和丝状型中发现了75种不同的蛋白质，其中10种蛋白质在酵母型中上调，3种在丝状型中上调。蛋白质2900和3151仅在酵母型中表达，蛋白质13151和13285仅在丝状型中表达。
结论
SELDI技术可以识别马尔尼菲青霉菌丝状型和酵母型之间低分子量表达蛋白的差异。
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