Davis F B, Smith T J, Davis P J, Lawrence W D, Ryan A J, Farrell M O, Blas S D
Department of Medicine, State University of New York, Buffalo School of Medicine and Biomedical Sciences, NY.
Biochem J. 1991 Jan 15;273(Pt 2)(Pt 2):489-92. doi: 10.1042/bj2730489.
The interaction was examined in vitro of retinoic acid and thyroid hormone with rabbit reticulocyte Ca2(+)-ATPase. L-Thyroxine (T4) (0.1 nM) stimulated female-source Ca2(+)-ATPase activity (+21%; P less than 0.03) and inhibited male-source enzyme (-20%; P less than 0.05). Addition of retinoic acid (10 nM-1 microM) did not influence T4-inhibitable male-source Ca2(+)-ATPase, but caused a 52% loss of T4 effect on the female-source enzyme. Incubation of female-source membranes with testosterone caused the enzyme response to T4 and retinoic acid to become that of male-source membranes, and the male-source enzyme response was converted into the 'female' pattern by exposure to 17 beta-oestradiol. We postulate that a membrane-associated sex-steroid-dependent factor imparts a gender-specific interaction of thyroid hormone and retinoic acid on Ca2(+)-ATPase, and that ultimately the factor is shed during erythrocyte maturation.
研究了视黄酸和甲状腺激素与兔网织红细胞Ca2(+)-ATP酶在体外的相互作用。L-甲状腺素（T4）（0.1 nM）刺激了来自雌性的Ca2(+)-ATP酶活性（增加21%；P<0.03），并抑制了来自雄性的酶活性（降低20%；P<0.05）。添加视黄酸（10 nM - 1 microM）对T4可抑制的雄性来源Ca2(+)-ATP酶没有影响，但导致T4对雌性来源酶的作用丧失了52%。用睾酮孵育雌性来源的膜会使该酶对T4和视黄酸的反应变为雄性来源膜的反应，而雄性来源酶的反应通过暴露于17β-雌二醇而转变为“雌性”模式。我们推测，一种与膜相关的、依赖于性类固醇的因子赋予了甲状腺激素和视黄酸对Ca2(+)-ATP酶的性别特异性相互作用，并且最终该因子在红细胞成熟过程中脱落。
