基因筛选的艺术与设计：玉米

The art and design of genetic screens: maize.

作者信息

Candela Héctor, Hake Sarah

机构信息

Plant Gene Expression Center, 800 Buchanan Street, Albany, California 94710, USA.

出版信息

Nat Rev Genet. 2008 Mar;9(3):192-203. doi: 10.1038/nrg2291.

DOI:10.1038/nrg2291

PMID:18250623

Abstract

Maize (Zea mays) is an excellent model for basic research. Genetic screens have informed our understanding of developmental processes, meiosis, epigenetics and biochemical pathways--not only in maize but also in other cereal crops. We discuss the forward and reverse genetic screens that are possible in this organism, and emphasize the available tools. Screens exploit the well-studied behaviour of transposon systems, and the distinctive chromosomes allow an integration of cytogenetics into mutagenesis screens and analyses. The imminent completion of the maize genome sequence provides the essential resource to move seamlessly from gene to phenotype and back.

摘要

玉米（Zea mays）是基础研究的优秀模式生物。遗传筛选有助于我们理解发育过程、减数分裂、表观遗传学和生化途径——不仅在玉米中，也在其他谷类作物中。我们讨论了在这种生物体中可行的正向和反向遗传筛选，并强调了可用的工具。筛选利用了转座子系统经过充分研究的行为，其独特的染色体使得细胞遗传学能够整合到诱变筛选和分析中。玉米基因组序列即将完成，这提供了从基因到表型再返回的无缝转换所需的重要资源。