Chong Leelyn, Su Huihui, Liu Yingpeng, Zheng Lingling, Tao Lianying, Bie Hai, Xiao Di, Yang Yang, Zhang Wanjun, Zhang Jing, Ren Jifei, Liu Huafeng, Ren Zhenzhen, Chen Yanhui, Xi Zhangying, Li Chengwei, Ku Lixia
National Key Laboratory of Wheat and Maize Crop Science and Key Laboratory of Regulating and Controlling Crop Growth and Development Ministry of Education, College of Agronomy, Henan Agricultural University, Zhengzhou, 450046, Henan, China.
Zhengzhou Agricultural Science and Technology Research Institute, Zhengzhou, China.
Theor Appl Genet. 2025 Mar 27;138(4):83. doi: 10.1007/s00122-025-04878-z.
We created the Zheng58 EMS Mutation Library, which provides a valuable resource for future investigations into the functions and roles of specific genes in maize physiology and development. Understanding the genetic variations present within maize is fundamental to improving maize breeding programs and developing crops with desirable traits. In this work, the Gene-Indexed Ethyl Methanesulfonate (EMS) Mutation Library of Zheng58 in maize was created to accelerate maize genetics research. By chemically inducing mutations in the Zheng58 maize inbred line using (EMS), 426 M EMS lines were generated, of which 185 exhibited heritable phenotypic changes. Coupling with high throughput sequencing techniques, over two million mutations, encompassing single nucleotide polymorphisms (SNPs) and small insertions and deletions (InDels), were subsequently identified. Functional annotation of mutation sites further indicated that a significant number of mutations influences important cellular processes, including translation termination (8,279), splice site disruption leading to mis-splicing (9,504), and missense mutations affecting protein-coding sequences (52,494). Therefore, the Gene-Indexed EMS Mutation Library of maize Zheng58 provides a valuable resource for future investigations into the functions and roles of specific genes in maize physiology and development.
我们创建了郑58 EMS突变文库，该文库为今后研究特定基因在玉米生理和发育中的功能及作用提供了宝贵资源。了解玉米中存在的遗传变异对于改进玉米育种计划和培育具有理想性状的作物至关重要。在这项工作中，创建了玉米郑58的基因索引甲基磺酸乙酯（EMS）突变文库，以加速玉米遗传学研究。通过使用甲基磺酸乙酯（EMS）对郑58玉米自交系进行化学诱变，产生了426个M EMS株系，其中185个表现出可遗传的表型变化。结合高通量测序技术，随后鉴定出超过200万个突变，包括单核苷酸多态性（SNP）和小插入缺失（InDel）。突变位点的功能注释进一步表明，大量突变影响重要的细胞过程，包括翻译终止（8279个）、导致错误剪接的剪接位点破坏（9504个）以及影响蛋白质编码序列的错义突变（52494个）。因此，玉米郑58的基因索引EMS突变文库为今后研究特定基因在玉米生理和发育中的功能及作用提供了宝贵资源。
