通过两步草氨酸盐琼脂糖亲和层析法从小鼠睾丸中快速纯化乳酸脱氢酶X。
Rapid purification of lactate dehydrogenase X from mouse testes by two steps of affinity chromatography on oxamate-sepharose.
作者信息
Spielmann H, Eibs H G, Mentzel C
出版信息
Experientia. 1976 Aug 15;32(8):1085-6. doi: 10.1007/BF01933984.
PMID:182529
Abstract
At concentrations of 200 muM NADH and 0.5 M NaCl LDH-X is separated from the other LDH isozymes of mouse testes on oxamate-sepharose. In a second step LDH-X is bound to the same matrix at lower NADH and NaCl concentrations and the pure enzyme can subsequently be eluted.
摘要
在200微摩尔NADH和0.5摩尔/升氯化钠的浓度下，乳酸脱氢酶-X（LDH-X）在草氨酸-琼脂糖上可与小鼠睾丸的其他乳酸脱氢酶同工酶分离。第二步，在较低的NADH和氯化钠浓度下，LDH-X与同一基质结合，随后可洗脱得到纯酶。
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