Somerman M J, Sauk J J, Foster R A, Norris K, Dickerson K, Argraves W S
Department of Periodontics, University of Maryland Dental School, Baltimore.
J Periodontal Res. 1991 Jan;26(1):10-6. doi: 10.1111/j.1600-0765.1991.tb01620.x.
Considerable research effort has been directed at preparing root surfaces in a fashion that would promote cell attachment leading to periodontal regeneration; however, no methods have proven to be clinically predictable. Identification of attachment protein(s) associated with the root surface matrix of cementum may prove valuable for developing effective clinical treatments. In this study cementum proteins were extracted from bovine and human teeth by sequential chaotropic extraction using guanidine followed by guanidine/EDTA. The guanidine/EDTA extract, but not guanidine extract, was found to promote attachment of fibroblasts. This attachment activity was inhibitable with synthetic peptide containing the attachment sequence arginine-glycine-aspartic acid (RGD). Fractionation of the guanidine/EDTA extract revealed several fractions with attachment activity. Immunoblot analysis demonstrated that two of these fractions contain the bone-associated RGD containing attachment protein, bone sialoprotein-II (BSP-II). In addition, attachment activity was also noted in other fractions that could not be attributed to BSP-II or fibronectin. These studies indicate that a component of the attachment activity of cementum is likely to be due to BSP-II and that cementum contains additional, as yet undetermined, attachment proteins.
相当多的研究致力于以促进细胞附着从而实现牙周再生的方式处理牙根表面；然而，尚无方法被证明在临床上具有可预测性。鉴定与牙骨质根面基质相关的附着蛋白可能对开发有效的临床治疗方法具有重要价值。在本研究中，通过使用胍随后用胍/乙二胺四乙酸（EDTA）进行连续的离液序列高盐提取，从牛牙和人牙中提取牙骨质蛋白。发现胍/EDTA提取物而非胍提取物能促进成纤维细胞的附着。这种附着活性可被含有附着序列精氨酸-甘氨酸-天冬氨酸（RGD）的合成肽抑制。对胍/EDTA提取物进行分级分离后发现了几个具有附着活性的级分。免疫印迹分析表明，其中两个级分含有与骨相关的含RGD附着蛋白，即骨唾液蛋白-II（BSP-II）。此外，在其他不能归因于BSP-II或纤连蛋白的级分中也观察到了附着活性。这些研究表明，牙骨质附着活性的一个组成部分可能归因于BSP-II，并且牙骨质还含有其他尚未确定的附着蛋白。
