Li Shi Zhao, Cao Mo Ju, Rong Ting Zhao, Pan Guang Tang, Tang Qi Lin, Zhu Ying Guo
Maine Research Institute of Sichuan Agricultural University/Key Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Ya'an 625014, Sichuan, China.
Fen Zi Xi Bao Sheng Wu Xue Bao. 2007 Oct;40(5):359-64.
In order to understand the cytological mechanism of pollen abortion of genetic male sterile mutant induced by space flight in maize, the sister cross population were used for sterility analysis and cytological observation. Intact anther observation, isolated cells observation and paraffin section were adopted in this research. The results showed that pollen abortion occured mostly in dyad stage of meiosis in genetic male sterile mutant. The dyad were degenerated with abnormal shape. In late anther developing stage, the tapetal cells were giant vacuolated and delayed degeneration. The pollen mother cells (PMC) began to dissolve and degenerate in a few anther before meiosis.
为了解玉米航天诱变遗传雄性不育突变体花粉败育的细胞学机制,利用姊妹交群体进行育性分析和细胞学观察。本研究采用完整花药观察、游离细胞观察和石蜡切片法。结果表明,遗传雄性不育突变体的花粉败育大多发生在减数分裂的二分体时期。二分体形态异常并发生退化。在花药发育后期,绒毡层细胞出现大液泡化且退化延迟。花粉母细胞在减数分裂前少数花药中就开始溶解和退化。