Rydh M, Malm M, Jernbeck J, Dalsgaard C J
Department of Anatomy, Karolinska Institute, Stockholm, Sweden.
Plast Reconstr Surg. 1991 Mar;87(3):419-22. doi: 10.1097/00006534-199103000-00003.
The innervation pattern of port-wine stains was investigated using indirect immunohistochemistry with antibodies to protein gene product 9.5 (PGP 9.5), neuron-specific enolase (NSE), calcitonin gene-related peptide (CGRP), and neurofilament (NF). The pathologically dilated vessels in the middle and deep dermis were found to have defective innervation with only single or no nerve fibers in their vicinity, while other structures in the skin showed a normal density of fibers. NSE- and PGP-like immunoreactive (-LI) nerve fibers were observed innervating vessels with a normal morphology and other structures in the skin, such as sweat glands and hair follicles, as free nerve endings and in nerve bundles. The nerve bundles were often seen to pass the ectatic vessels without giving off any branches. CGRP-LI nerve fibers were detected running toward epidermis, whereas no fibers were found around the ectatic vessels. NF-LI fibers were seen innervating normal vessels in dermis, while in relation to the dilated vessels, no or only occasional fibers were observed. The lack of innervation may be of importance for the development of the disease as a result of decreased tonus of the vessels and/or a loss of neuronal trophic factors.
采用间接免疫组织化学法，使用抗蛋白基因产物9.5（PGP 9.5）、神经元特异性烯醇化酶（NSE）、降钙素基因相关肽（CGRP）和神经丝（NF）的抗体，研究葡萄酒色斑的神经支配模式。发现真皮中层和深层病理扩张的血管神经支配存在缺陷，其附近仅有单根或无神经纤维，而皮肤中的其他结构显示纤维密度正常。观察到NSE和PGP样免疫反应性（-LI）神经纤维以游离神经末梢和神经束的形式支配形态正常的血管以及皮肤中的其他结构，如汗腺和毛囊。经常可见神经束穿过扩张的血管而不发出任何分支。检测到CGRP-LI神经纤维向表皮延伸，而在扩张血管周围未发现纤维。观察到NF-LI纤维支配真皮中的正常血管，而对于扩张的血管，未观察到或仅偶尔观察到纤维。神经支配的缺乏可能由于血管张力降低和/或神经元营养因子丧失而对疾病的发展具有重要意义。
