Wada S, Okuno M, Mohri H
Department of Biology, College of Arts and Sciences, University of Tokyo, Japan.
Biochem Biophys Res Commun. 1991 Feb 28;175(1):173-8. doi: 10.1016/s0006-291x(05)81216-1.
In order to clarify the role of the inner arms of the axoneme in sperm flagellar movement, we prepared an ATPase fraction (12S) from the outer arm-depleted axonemes of sea urchin sperm flagella. When both arm-depleted axonemes were incubated with the 12S ATPase, they exhibited the sliding disintegration of outer doublet microtubules. Electron microscopy revealed that the ATPase rebound to the original inner arm sites of the axoneme. Therefore, it is quite likely that the 12S ATPase is one of the components of the inner arms. We referred to it as "inner arm dynein".
为了阐明轴丝内臂在精子鞭毛运动中的作用，我们从海胆精子鞭毛的外臂缺失轴丝中制备了一种ATP酶组分（12S）。当将双臂缺失的轴丝与12S ATP酶一起孵育时，它们表现出外侧双联微管的滑动解体。电子显微镜显示，ATP酶重新结合到轴丝原来的内臂部位。因此，12S ATP酶很可能是内臂的组成成分之一。我们将其称为“内臂动力蛋白”。
