Van der Pouw-Kraan T, Van Kooten C, Van Oers R, Aarden L A
Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Amsterdam.
Eur J Immunol. 1991 Feb;21(2):385-90. doi: 10.1002/eji.1830210220.
A solid-phase-coupled anti-CD3 T cell activation system was used to study the regulation of human IgE production in vitro. Using 5000 peripheral blood lymphocytes from healthy donors, containing 10%-20% B lymphocytes and no monocytes. IgE was produced very efficiently on a per cell basis. A key observation was that apart from interleukin (IL) 4, human transferrin was essential for IgE production. Furthermore it was found that IgE was produced at low densities only; at higher cell concentrations IgE production was completely abrogated, whereas IgM production increased with increasing cell density. This inhibition at higher cell densities is probably mediated by IL2. Addition of low amounts (6 U/ml) of IL2 strongly enhanced IgE and IgM production at low cell densities, but higher concentrations of IL2 (50 U/ml) were strongly inhibitory for IgE production.
采用固相偶联抗CD3 T细胞激活系统在体外研究人IgE产生的调控。使用来自健康供体的5000个外周血淋巴细胞，其中B淋巴细胞占10%-20%，且无单核细胞。以单个细胞为基础，IgE产生效率很高。一个关键的观察结果是，除了白细胞介素（IL）4外，人转铁蛋白对于IgE的产生也是必不可少的。此外，还发现仅在低密度时产生IgE；在较高细胞浓度下，IgE产生完全被消除，而IgM产生则随细胞密度增加而增加。这种在较高细胞密度下的抑制作用可能由IL2介导。添加少量（6 U/ml）IL2可在低细胞密度下强烈增强IgE和IgM的产生，但较高浓度的IL2（50 U/ml）对IgE产生具有强烈抑制作用。
