Damak Nahla, Bouaziz Mohamed, Ayadi Mohamed, Sayadi Sami, Damak Mohamed
Laboratoire de Chimie des Substances Naturelles, Faculté des Sciences de Sfax, B.P. 802, 3038 Sfax, Tunisia.
J Agric Food Chem. 2008 Mar 12;56(5):1560-6. doi: 10.1021/jf072273k. Epub 2008 Feb 8.
Maturity is one of the most important factors associated with the quality evaluation of fruit and vegetables. This work aims to investigate the effect of the maturation process of the olive fruit on the phenolic fraction and fatty acid of irrigated Chétoui cultivar. The phenolic composition was studied by using reverse-phase high-performance liquid chromatography followed by LC-MS and GC-MS analyses and fatty acids by GC. Oleuropein was the major phenolic compound at all stages of ripeness. Unexpectedly, both phenolic compounds hydroxytyrosol and oleuropein exhibited the same trends during maturation. Indeed, the oleuropein levels decreased during the ripening process and were not inversely correlated with the concentrations of hydroxytyrosol. The antioxidant capacity of olive extracts was evaluated by measuring the radical scavenging effect on 1,1-diphenyl-2-picrylhydrazyl and the beta-carotene linoleate model system. The IC 50 and AAC values of the olive extracts decreased from 3.68 to 1.61 microg/mL and from 645 to 431, respectively. There was a correlation between the antioxidant activity and the oleuropein concentration. The fatty acid composition was quantified in olive fruit during maturation and showed that fatty acids were characterized by the highest level of oleic acid, which reached 65.2%.
成熟度是与水果和蔬菜质量评估相关的最重要因素之一。本研究旨在探究橄榄果实成熟过程对灌溉种植的谢图伊品种酚类成分和脂肪酸的影响。采用反相高效液相色谱法,随后进行液相色谱 - 质谱联用和气相色谱 - 质谱联用分析来研究酚类成分,通过气相色谱法分析脂肪酸。橄榄苦苷是成熟各阶段的主要酚类化合物。出乎意料的是,酚类化合物羟基酪醇和橄榄苦苷在成熟过程中呈现相同趋势。实际上,橄榄苦苷水平在成熟过程中下降,且与羟基酪醇浓度并非呈负相关。通过测定对1,1 - 二苯基 - 2 - 苦基肼自由基清除效果以及β - 胡萝卜素亚油酸酯模型体系来评估橄榄提取物的抗氧化能力。橄榄提取物的半数抑制浓度(IC50)值和抗氧化活性系数(AAC)值分别从3.68微克/毫升降至1.61微克/毫升,从645降至431。抗氧化活性与橄榄苦苷浓度之间存在相关性。对成熟过程中的橄榄果实脂肪酸组成进行了定量分析,结果表明脂肪酸的特征是油酸含量最高,达到了65.2%。