Johnson Brandy J, Delehanty James B, Lin Baochuan, Ligler Frances S
Center for Bio/Molecular Science & Engineering, Naval Research Laboratory, Washington, D.C. 20375-5348, USA.
Anal Chem. 2008 Mar 15;80(6):2113-7. doi: 10.1021/ac7024128. Epub 2008 Feb 8.
Proanthocyanidins (PACs) are an abundant class of compounds found in a variety of plant materials. Here we demonstrate the application of these materials as capture molecules for the removal of bacterial lipopolysaccharide (LPS) from solution. PACs from whole cranberries, grape juice, black tea, and cranberry juice were purified and immobilized onto thiol-activated Sepharose beads. This material was used in pull-down type assays for the capture of LPS. The binding of LPS by PACs has been shown to compete with that of polymyxin B which is known to bind the lipid A component of LPS. Assays conducted in the presence of lipid A verified that at least some component of the LPS binding activity of the PACs is via the lipid A moiety. Molar comparison of polymyxin B to proanthocyanidins indicated that the Sepharose immobilized PACs have a binding affinity for LPS similar to that of polymyxin B.
原花青素（PACs）是一类在多种植物材料中大量存在的化合物。在此，我们展示了这些材料作为捕获分子从溶液中去除细菌脂多糖（LPS）的应用。从全蔓越莓、葡萄汁、红茶和蔓越莓汁中提取的PACs被纯化并固定在硫醇活化的琼脂糖珠上。这种材料用于下拉式实验以捕获LPS。已表明PACs与LPS的结合能与多粘菌素B竞争，已知多粘菌素B能结合LPS的脂质A成分。在脂质A存在下进行的实验证实，PACs的LPS结合活性至少部分是通过脂质A部分实现的。多粘菌素B与原花青素的摩尔比较表明，固定在琼脂糖上的PACs对LPS的结合亲和力与多粘菌素B相似。
