Coque Teresa M, Novais Angela, Carattoli Alessandra, Poirel Laurent, Pitout Johann, Peixe Luísa, Baquero Fernando, Cantón Rafael, Nordmann Patrice
Hospital Universitario Ramón y Cajal, Madrid, Spain.
Emerg Infect Dis. 2008 Feb;14(2):195-200. doi: 10.3201/eid1402.070350.
We analyzed 43 CTX-M-15-producing Escherichia coli isolates and 6 plasmids encoding the blaCTX-M-15 gene from Canada, India, Kuwait, France, Switzerland, Portugal, and Spain. Most isolates belonged to phylogroups B2 (50%) and D (25%). An EC-B2 strain of clonal complex sequence type (ST) 131 was detected in all countries; other B2 isolates corresponded to ST28, ST405, ST354, and ST695 from specific areas. EC-D strains were clonally unrelated but isolates from 3 countries belonged to ST405. All CTX-M-15 plasmids corresponded to IncFII group with overrepresentation of 3 HpaI-digested plasmid DNA profiles (A, B and C; 85-120kb, similarity > or =70%). Plasmid A was detected in EC-B2 strains (ST131, ST354, or ST405), plasmid C was detected in B2 and D strains, and plasmid B was confined to worldwide-disseminated ST131. Most plasmids contained blaOXA-1, aac(6')-Ib-cr, and blaTEM-1. Worldwide dissemination of CTX-M-15 seems to be determined by clonal complexes ST131 and ST405 and multidrug-resistant IncFII plasmids.
我们分析了来自加拿大、印度、科威特、法国、瑞士、葡萄牙和西班牙的43株产CTX-M-15的大肠杆菌分离株以及6个编码blaCTX-M-15基因的质粒。大多数分离株属于B2（50%）和D（25%）系统发育群。在所有国家均检测到克隆复合序列类型（ST）131的EC-B2菌株；其他B2分离株对应于特定地区的ST28、ST405、ST354和ST695。EC-D菌株在克隆上不相关，但来自3个国家的分离株属于ST405。所有CTX-M-15质粒均对应于IncFII组，3种HpaI酶切质粒DNA图谱（A、B和C；85 - 120kb，相似度≥70%）出现频率过高。质粒A在EC-B2菌株（ST131、ST354或ST405）中检测到，质粒C在B2和D菌株中检测到，质粒B局限于全球传播的ST131。大多数质粒含有blaOXA-1、aac(6')-Ib-cr和blaTEM-1。CTX-M-15的全球传播似乎由克隆复合体ST131和ST405以及多重耐药的IncFII质粒决定。
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