解嘌呤梭菌甘氨酸还原酶复合体的硒蛋白A组分：基因的核苷酸序列表明硒代半胱氨酸由UGA编码。
Selenoprotein A component of the glycine reductase complex from Clostridium purinolyticum: nucleotide sequence of the gene shows that selenocysteine is encoded by UGA.
作者信息
Garcia G E, Stadtman T C
机构信息
Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892.
出版信息
J Bacteriol. 1991 Mar;173(6):2093-8. doi: 10.1128/jb.173.6.2093-2098.1991.
Abstract
The gene encoding the selenoprotein A component of glycine reductase was isolated from Clostridium purinolyticum. The nucleotide sequence of this gene (grdA) was determined. The opal termination codon (TGA) was found in-frame at the position corresponding to the location of the selenocysteine residue in the gene product. A comparison of the nucleotide sequences and secondary mRNA structures corresponding to the selenoprotein A gene and the fdhF gene of Escherichia coli formate dehydrogenase shows that there is a similar potential for regulation of the specific insertion of selenocysteine at the UGA codon.
摘要
从解嘌呤梭菌中分离出编码甘氨酸还原酶硒蛋白A组分的基因。测定了该基因（grdA）的核苷酸序列。在与基因产物中硒代半胱氨酸残基位置相对应的位置，发现了琥珀终止密码子（TGA）处于读码框内。对大肠杆菌甲酸脱氢酶的硒蛋白A基因和fdhF基因对应的核苷酸序列及二级mRNA结构进行比较，结果表明，在UGA密码子处，硒代半胱氨酸的特异性插入具有相似的调控潜力。
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