Castelletto Valeria, Hamley Ian W, Clifton Luke A, Green Rebecca J
School of Chemistry, Food Biosciences and Pharmacy, The University of Reading, P.O. Box 226, Whiteknights, Reading, UK.
Biophys Chem. 2008 Apr;134(1-2):34-8. doi: 10.1016/j.bpc.2008.01.004. Epub 2008 Jan 17.
In this work we study the colloidal osmotic pressure (COP) and aggregate shape in phosphate saline buffer solutions (pH 7.4) containing bovine serum albumin (BSA), poly(ethylene glycol) lipid (PEG(2000)-PE) and Dextran (Dx). Dx was added to the BSA/PEG(2000)-PE system in order to increase the COP of the solution to levels comparable to the COP of healthy adults, with the aim of using the solution as a blood COP regulator. Dynamic light scattering and small angle X-ray scattering results shown the formation of BSA/PEG(2000)-PE/Dx aggregates in the solution. Osmometry results shown that the addition of Dx to the BSA/PEG(2000)-PE system could successfully increase the COP, through the formation of BSA/PEG(2000)-PE/Dx aggregates. The BSA/PEG(2000)-PE/Dx solutions attained COP=15 mm Hg, representing 60% of COP measured for healthy adults.
在这项工作中，我们研究了含有牛血清白蛋白（BSA）、聚乙二醇脂质（PEG(2000)-PE）和葡聚糖（Dx）的磷酸盐缓冲溶液（pH 7.4）中的胶体渗透压（COP）和聚集体形状。将Dx添加到BSA/PEG(2000)-PE体系中，以将溶液的COP提高到与健康成年人的COP相当的水平，目的是将该溶液用作血液COP调节剂。动态光散射和小角X射线散射结果表明溶液中形成了BSA/PEG(2000)-PE/Dx聚集体。渗透压测定结果表明，向BSA/PEG(2000)-PE体系中添加Dx可通过形成BSA/PEG(2000)-PE/Dx聚集体成功提高COP。BSA/PEG(2000)-PE/Dx溶液的COP达到15 mmHg，占健康成年人测得的COP的60%。
