Simms H H, D'Amico R, Monfils P, Burchard K W
Department of Surgery, Rhode Island Hospital, Providence 02903.
J Lab Clin Med. 1991 Mar;117(3):241-9.
We investigated the effects of untreated intraabdominal sepsis on polymorphonuclear leukocyte (PMN) candicidal activity. Two groups of swine were studied. Group I (n = 6) underwent sham laparotomy, group II (n = 7) underwent cecal ligation and incision. Untreated intraabdominal sepsis resulted in a progressive decrease in PMN candicidal activity. Concomitant rosetting and phagocytosis assays demonstrated a decrease in both the attachment and phagocytosis of Candida albicans opsonized with both normal and septic swine serum by PMNs in group II. Iodine 125-labeled swine immunoglobulin G (IgG) and fluorescein isothioalanate (FITC)-labeled swine IgG were used to investigate Fc gamma receptor ligand interactions. Scatchard analyses demonstrated a progressive decline in both the binding affinity constant and number of IgG molecules bound per PMN. Stimulation of the oxidative burst markedly reduced 125I-labeled IgG binding in both group I and group II, with a greater decrement being seen in animals with intraabdominal sepsis. Further, in group II, PMN recycling of the Fc gamma receptor to the cell surface after generation of the oxidative burst was reduced by postoperative day 4. Binding of monoclonal antibodies to Fc gamma receptor II, but not Fc gamma receptor I/III markedly reduced intracellular candicidal activity. Immunofluorescence studies revealed a homogeneous pattern of FITC-IgG uptake by nearly all group I PMNs, whereas by postoperative day 8 a substantial number of PMNs from group II failed to internalize the FITC-IgG. These studies suggest that untreated intraabdominal sepsis reduces PMN candicidal activity and that this is due, in part, to altered PMN Fc gamma receptor ligand interactions.
我们研究了未经治疗的腹腔内脓毒症对多形核白细胞（PMN）杀念珠菌活性的影响。研究了两组猪。第一组（n = 6）接受假剖腹手术，第二组（n = 7）接受盲肠结扎和切开术。未经治疗的腹腔内脓毒症导致PMN杀念珠菌活性逐渐降低。同时进行的玫瑰花结形成和吞噬试验表明，第二组中PMN对用正常猪血清和脓毒症猪血清调理的白色念珠菌的黏附及吞噬均减少。使用碘125标记的猪免疫球蛋白G（IgG）和异硫氰酸荧光素（FITC）标记的猪IgG来研究Fcγ受体配体相互作用。Scatchard分析表明，结合亲和力常数和每个PMN结合的IgG分子数量均逐渐下降。氧化爆发刺激显著降低了第一组和第二组中125I标记的IgG结合，腹腔内脓毒症动物的下降幅度更大。此外，在第二组中，术后第4天氧化爆发后Fcγ受体向细胞表面的PMN再循环减少。单克隆抗体与Fcγ受体II结合，但不与Fcγ受体I/III结合，显著降低细胞内杀念珠菌活性。免疫荧光研究显示，几乎所有第一组PMN对FITC-IgG的摄取呈均匀模式，而到术后第8天，第二组中有大量PMN未能内化FITC-IgG。这些研究表明，未经治疗的腹腔内脓毒症会降低PMN杀念珠菌活性，部分原因是PMN的Fcγ受体配体相互作用发生改变。
