[RAS 关联结构域家族基因 1A 启动子区域在食管鳞状细胞癌中的高甲基化及其意义]

[Hypermethylation of promoter region of RAS association domain family gene1A in esophageal squamous cell carcinoma and significance thereof].

作者信息

Cong De-gang, Wang Sheng-fa

机构信息

Department of Thoracic Surgery, Affiliated Hospital of Hangzhou Normal University, Hangzhou 310015, China.

出版信息

Zhonghua Yi Xue Za Zhi. 2007 Nov 6;87(41):2932-4.

PMID:18261310

Abstract

OBJECTIVE

To investigate the hypermethylation of the promoter region of RAS association domain family gene1A (RASSF1A) and its relationship with esophageal squamous cell carcinoma.

METHODS

Sixty-six patients with esophageal squamous carcinoma, 60 males and 6 females, aged 59 +/- 8 (44 - 76), underwent resection of the tumor. Methylation-specific PCR (MSP) was used to detect the hypermethylation of promoter region of RASSF1A in the carcinoma tissues and the adjacent normal tissues.

RESULTS

The hypermethylation rate of RASSF1A promoter in the tumor tissues was 48. 5% (32/66), significantly higher than that in the adjacent normal tissues (6.1%, 4/66, P < 0.05). The hypermethylation rate of RASSF1A promoter of the patients with lymph node metastasis was 61.1%, significantly higher than that of the patients without lymph node metastasis (33.3%, chi(2) = 5.055, P = 0.025). The hypermethylation rate of RASSF1A promoter in the esophageal squamous cell carcinoma at advanced stages (stages III - IV) was 69.2%, significantly higher than that in the esophageal squamous cell carcinoma at early stages (stages I - II, 35.0%, chi(2) = 7.392, P = 0.007). The hypermethylation rates of RASSF1A promoter in the high, moderate, and low differentiation tumors were 61.5% (16/26), 46.2% (12/26), and 28.6% (4/14) respectively without significant differences among them (chi(2) = 4.053, P = 0.132).

CONCLUSION

Abnormal methylation exists in the RASSF1A promoter in esophageal squamous cell carcinoma. The hypermethylation of RASSF1A promoter is related to lymph node metastasis and TNM stage.

摘要

目的

探讨RAS关联结构域家族基因1A（RASSF1A）启动子区域的高甲基化及其与食管鳞状细胞癌的关系。

方法

66例食管鳞状癌患者，男60例，女6例，年龄59±8（44 - 76）岁，均接受肿瘤切除术。采用甲基化特异性PCR（MSP）检测癌组织及癌旁正常组织中RASSF1A启动子区域的高甲基化情况。

结果

肿瘤组织中RASSF1A启动子的高甲基化率为48.5%（32/66），显著高于癌旁正常组织（6.1%，4/66，P < 0.05）。有淋巴结转移患者的RASSF1A启动子高甲基化率为61.1%，显著高于无淋巴结转移患者（33.3%，χ² = 5.055，P = 0.025）。晚期（Ⅲ - Ⅳ期）食管鳞状细胞癌中RASSF1A启动子的高甲基化率为69.2%，显著高于早期（Ⅰ - Ⅱ期）食管鳞状细胞癌（35.0%，χ² = 7.392，P = 0.007）。高、中、低分化肿瘤中RASSF1A启动子的高甲基化率分别为61.5%（16/26）、46.2%（12/26）和28.6%（4/14），差异无统计学意义（χ² = 4.053，P = 0.132）。