Vohra R, Thomson G J, Carr H M, Sharma H, Welch M, Walker M G
Department of Vascular Surgery, Manchester Royal Infirmary, U.K.
Eur J Vasc Surg. 1991 Feb;5(1):93-103. doi: 10.1016/s0950-821x(05)80934-x.
Lining the luminal surface of small diameter vascular prostheses with living endothelial cells reduces thrombogenicity, decreases infection and improves patency. In vitro adherence and kinetics studies of adult human endothelial cell seeded Polytetrafluoroethylene (ePTFE) and Gelatin impregnated Dacron (Gelseal) were performed. Endothelial cell adherence on ePTFE and Gelseal coated with collagen IV, fibronectin and preclot matrices was compared. Untreated ePTFE and Gelseal were also used. Ten graft segments in each group coated with these matrices were incubated with radio-labelled adult human endothelial cells for 30, 60 and 90 min. Labelled endothelial cells seeded in supra-confluent densities on fibronectin coated ePTFE and Gelseal grafts were used for kinetic studies. Resultant endothelial cell monolayers were then exposed to varying shear stress at flow rates of 200 and 300 ml/min in an artificial flow circuit. Endothelial cell attachment to untreated grafts was poor and a suitable matrix significantly improved adherence with fibronectin and preclot but less so with collagen. A 30 min incubation was sufficient for optimum cell attachment. Cell adherence to ePTFE was significantly better than Gelseal. Scanning electron micrographs (SEM) of ePTFE showed preferential attachment to the nodes whilst on Gelseal, cells conformed to Dacron fibres at different levels and directions. Rapidly formed endothelial cell monolayers on ePTFE and Gelseal grafts resisted shear stress of flow with significant cell retention at 2 h. There was patchy coverage of both grafts with evidence of bridging of gaps between individual fibres in Gelseal.
用活的内皮细胞覆盖小口径血管假体的管腔表面可降低血栓形成性、减少感染并提高通畅率。对接种了成人内皮细胞的聚四氟乙烯（ePTFE）和明胶浸渍涤纶（Gelseal）进行了体外黏附及动力学研究。比较了内皮细胞在涂有IV型胶原、纤连蛋白和预凝块基质的ePTFE和Gelseal上的黏附情况。还使用了未处理的ePTFE和Gelseal。每组中十个涂有这些基质的移植物段与放射性标记的成人内皮细胞孵育30、60和90分钟。接种在纤连蛋白包被的ePTFE和Gelseal移植物上的超汇合密度的标记内皮细胞用于动力学研究。然后在人工流动回路中，使所得的内皮细胞单层暴露于200和300毫升/分钟流速下的不同剪切应力。内皮细胞与未处理移植物的附着较差，合适的基质显著改善了与纤连蛋白和预凝块的黏附，但与胶原的黏附改善较少。30分钟的孵育足以实现最佳细胞附着。细胞对ePTFE的黏附明显优于Gelseal。ePTFE的扫描电子显微镜图像（SEM）显示优先附着于节点，而在Gelseal上，细胞在不同水平和方向上与涤纶纤维贴合。ePTFE和Gelseal移植物上快速形成的内皮细胞单层抵抗流动的剪切应力，在2小时时有显著的细胞保留。两种移植物均有斑片状覆盖，Gelseal中有单个纤维间间隙桥接的证据。
