Anraku Y, Mizuta H, Sei A, Kudo S, Nakamura E, Senba K, Takagi K, Hiraki Y
Department of Orthopaedic and Neuro-Musculoskeletal Surgery, Faculty of Medical and Pharmaceutical Sciences, Kumamoto University, Kumamoto 860-8556, Japan.
Osteoarthritis Cartilage. 2008 Aug;16(8):961-4. doi: 10.1016/j.joca.2007.12.009. Epub 2008 Feb 11.
The aim of this study is to develop a rat model of full-thickness articular cartilage defects that is suitable for detailed molecular analyses of the regenerative repair of cartilage.
The V-shaped full-thickness defects (width: 0.7 mm; depth: 0.8 mm; and length: 4mm) were created in the femoral patellar groove of 6 weeks old male rats using a custom-built twin-blade device. Prior to starting the repair experiments, our device was examined for its accuracy and reliability in generating defects. Then, the time course of the repair response in these cartilage defects was examined using a semi-quantitative histological grading scale. The expression of chondrogenic differentiation markers in the reparative regions was examined with immunohistochemistry and in situ hybridization.
Our device creates full-thickness articular cartilage defects uniformly. In these defects, undifferentiated mesenchymal cells filled the defect cavities (4 days) and initiated chondrogenic differentiation at the center of the defect (7 days). Cartilage formation was observed in the same region (2 weeks). Finally, hyaline-like articular cartilage and subchondral bone layers were reconstituted in their appropriate locations (4 weeks).
We have successfully developed a rat model containing identically sized full-thickness defects of articular cartilage that can undergo chondrogenic repair in a reproducible fashion.
本研究旨在建立一种适合对软骨再生修复进行详细分子分析的大鼠全层关节软骨缺损模型。
使用定制的双刃器械在6周龄雄性大鼠的股骨髌沟处制造V形全层缺损（宽度：0.7毫米；深度：0.8毫米；长度：4毫米）。在开始修复实验之前，检测了我们的器械在制造缺损方面的准确性和可靠性。然后，使用半定量组织学分级量表检查这些软骨缺损修复反应的时间进程。用免疫组织化学和原位杂交法检测修复区域中软骨分化标志物的表达。
我们的器械能均匀地制造全层关节软骨缺损。在这些缺损中，未分化的间充质细胞填充缺损腔（4天）并在缺损中心开始软骨分化（7天）。在同一区域观察到软骨形成（2周）。最后，在适当位置重建了透明样关节软骨和软骨下骨层（4周）。
我们成功建立了一种大鼠模型，其包含大小相同的全层关节软骨缺损，且能以可重复的方式进行软骨形成修复。
