黏粒和质粒克隆的纯化
Purification of cosmid and plasmid clones.
作者信息
Weis J H
机构信息
Harvard Medical School, Boston, Massachusetts, USA.
出版信息
Curr Protoc Mol Biol. 2001 May;Chapter 6:Unit6.6. doi: 10.1002/0471142727.mb0606s17.
PMID:18265261
Abstract
Cosmid- or plasmid-bearing colonies that are identified by hybridization are purified by spreading the cosmids or plasmids on an agar plate and repeating the colony hybridization.
摘要
通过杂交鉴定出的携带黏粒或质粒的菌落，可通过将黏粒或质粒铺在琼脂平板上并重复菌落杂交来进行纯化。
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