Elbing Karen, Brent Roger
Clark & Elbing LLP, Boston, Massachusetts, USA.
Curr Protoc Mol Biol. 2002 Aug;Chapter 1:Unit 1.2. doi: 10.1002/0471142727.mb0102s59.
The procedure for inoculating overnight (starter) cultures of E. coli from a single colony is described along with considerations for growing larger cultures. Also included are two methods for monitoring cell growth using a spectrophotometer or a hemacytometer.
本文描述了从单个菌落接种大肠杆菌过夜(起始)培养物的步骤,以及扩大培养时的注意事项。此外,还介绍了使用分光光度计或血细胞计数器监测细胞生长的两种方法。