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Use of monoclonal antibodies for expression cloning.

作者信息

Hollenbaugh Diane, Aruffo Alejandro, Jones Bryan, Linsley Peter

机构信息

Bristol-Myers Squibb, Seattle, Washington, USA.

出版信息

Curr Protoc Mol Biol. 2003 May;Chapter 6:Unit 6.11. doi: 10.1002/0471142727.mb0611s62.

DOI:10.1002/0471142727.mb0611s62
PMID:18265331
Abstract

This unit details the use of transient expression in mammalian cells to screen cDNA libraries with monoclonal antibodies (MAb) to isolate cDNA clones encoding cell-surface and intracellular proteins. The first protocol in this unit describes the cloning of cDNAs encoding cell-surface antigens. Several steps in this protocol involve transfection procedures that are described in greater detail elsewhere in this volume. The second protocol is a modification that facilitates isolation of cDNAs encoding antigens that are expressed intracellularly. Both protocols are designed for use with the expression vector CDM8, which contains a polylinker for subcloning double-stranded cDNA.

摘要

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