Ling Paul D, Huls Helen M
Baylor College of Medicine, Houston, Texas, USA.
Curr Protoc Mol Biol. 2005 May;Chapter 28:Unit 28.2. doi: 10.1002/0471142727.mb2802s70.
Recent advances in Genomics and Proteomics have necessitated a constant supply of DNA derived from specific genotypes. Lymphoblastoid cell lines (LCLs) are of great practical value as an unlimited source of stable genomic DNA and viable cells, which can be used to perform a variety of biochemical and molecular studies. LCLs are typically generated by infection of primary lymphocytes with Epstein-Barr virus (EBV). In this unit, we describe simple procedures for production of EBV, isolation of lymphocytes, EBV infection of lymphocytes, and isolation of the resulting LCLs.
基因组学和蛋白质组学的最新进展使得持续供应源自特定基因型的DNA成为必要。淋巴母细胞系（LCLs）作为稳定基因组DNA和活细胞的无限来源具有巨大的实用价值，可用于进行各种生化和分子研究。LCLs通常通过用爱泼斯坦-巴尔病毒（EBV）感染原代淋巴细胞产生。在本单元中，我们描述了生产EBV、分离淋巴细胞、淋巴细胞的EBV感染以及分离所得LCLs的简单程序。
