Tripathi M K, Mishra A S, Misra A K, Vaithiyanathan S, Prasad R, Jakhmola R C
Division of Animal Nutrition, Central Sheep and Wool Research Institute, Avikanagar, Rajasthan, India.
Lett Appl Microbiol. 2008 Mar;46(3):364-70. doi: 10.1111/j.1472-765X.2008.02320.x. Epub 2008 Feb 7.
Selection of white-rot fungi of bio-conversion of mustard straw (MS) into feed for ruminants.
Mustard straw was cultured with Ganoderma applanatum, Coriolus versicolor and Phanerochaete chrysosporium for solid-state fermentation at 35 degrees C from 7 to 63 days for delignification and for 21 days to study dry matter digestibility and protein enrichment. Lignin loss in fungus cultured straw varied between 100 and 470 g kg(-1) lignin. Delignification was higher between 7 and 28 days fermentation with C. versicolor. Among the three fungi P. chrysosporium was the most effective in degrading lignin for longer fermentation. In-vitro dry matter digestibility (IVDMD) and crude protein content was higher in C. versicolor cultured straw. Large quantity of straw was cultured by C. versicolor for 21 days, for in vivo evaluation. Mean pH and metabolites of rumen fermentation were not different while, pH and volatile fatty acid increased at 6 h postfermentation on cultured straw feeding. Cultured straw fermentation increased (P = 0.001) small holotricks and reduced (P = 0.005) large holotricks population. Fungus cultures straw did not improve microbial enzyme concentration.
Coriolus versicolor and P. chrysosporium were the promising fungus for MS bio-delignification.
Coriolus versicolor treated MS improved dry matter digestibility and protein content.
筛选能将芥菜秸秆生物转化为反刍动物饲料的白腐真菌。
将芥菜秸秆分别与树舌灵芝、云芝和黄孢原毛平革菌进行培养，在35℃下进行固态发酵7至63天以进行脱木质素处理，并发酵21天以研究干物质消化率和蛋白质富集情况。真菌培养秸秆中的木质素损失在100至470 g kg⁻¹木质素之间。云芝在发酵7至28天期间脱木质素效果更好。在三种真菌中，黄孢原毛平革菌在较长发酵时间内降解木质素最为有效。云芝培养的秸秆体外干物质消化率（IVDMD）和粗蛋白含量更高。用云芝培养了大量秸秆21天用于体内评估。瘤胃发酵的平均pH值和代谢产物没有差异，而在饲喂培养秸秆后6小时，pH值和挥发性脂肪酸增加。培养秸秆发酵使小型全毛虫数量增加（P = 0.001），大型全毛虫数量减少（P = 0.005）。真菌培养的秸秆并未提高微生物酶浓度。
云芝和黄孢原毛平革菌是芥菜秸秆生物脱木质素的有前景的真菌。
云芝处理的芥菜秸秆提高了干物质消化率和蛋白质含量。
