人滤泡树突状细胞：原位及悬浮状态下的分离与特性

Human follicular dendritic cells: isolation and characteristics in situ and in suspension.

作者信息

Parmentier H K, van der Linden J A, Krijnen J, van Wichen D F, Rademakers L H, Bloem A C, Schuurman H J

机构信息

Department of Internal Medicine, University Hospital, Utrecht, The Netherlands.

出版信息

Scand J Immunol. 1991 Apr;33(4):441-52. doi: 10.1111/j.1365-3083.1991.tb01793.x.

DOI:10.1111/j.1365-3083.1991.tb01793.x

PMID:1826796

Abstract

Follicular dendritic cells (FDC) in human tonsils, either in situ in follicular germinal centres or isolated from tissue, were characterized by immunohistochemical, enzyme cytochemical and electron microscopical methods. Using polyclonal and monoclonal antibodies, expression of DRC-1, Ki-M4, HLA-DR, CR1, C1q antigens, a macrophage marker, and surface IgG and IgM were found on isolated FDC and on FDC in situ. None of these reagents proved to be specific for FDC, e.g. the FDC-directed antibodies DRC-1 and Ki-M4 labelled B lymphocytes in cytofluorography. Enzyme cytochemical staining revealed activities of non-specific esterase, acid alpha-naphthylacetate esterase and ATPase in germinal centres and in freshly isolated FDC. Immunohistochemistry demonstrated a weak expression of CD4 by a fraction of isolated FDC, which was confirmed by two-colour immuno-staining and immuno-electron microscopy.

摘要

采用免疫组织化学、酶细胞化学及电子显微镜方法，对人扁桃体中的滤泡树突状细胞（FDC）进行了特征分析，这些细胞既存在于滤泡生发中心的原位组织中，也从组织中分离得到。利用多克隆和单克隆抗体，在分离的FDC及原位FDC上发现了DRC-1、Ki-M4、HLA-DR、CR1、C1q抗原、一种巨噬细胞标志物以及表面IgG和IgM的表达。这些试剂均未被证明对FDC具有特异性，例如，FDC定向抗体DRC-1和Ki-M4在细胞荧光术中标记了B淋巴细胞。酶细胞化学染色显示生发中心及新鲜分离的FDC中存在非特异性酯酶、酸性α-萘乙酸酯酶和ATP酶的活性。免疫组织化学表明，一部分分离的FDC弱阳性表达CD4，双色免疫染色和免疫电子显微镜证实了这一点。