Luo X C, Xie M Q, Zhu X Q, Li A X
School of Life Sciences, Sun Yat-sen University, 135 Xingang West Street, Haizhu District, Guangzhou, Guangdong Province 510275, People's Republic of China.
Parasitol Res. 2008 May;102(6):1269-75. doi: 10.1007/s00436-008-0904-9. Epub 2008 Feb 13.
A survey on the host range for the parasitic ciliate Cryptocaryon irritans was carried out among the major maricultured fish species in the Huizhou region of Guangdong Province in South China, and some characteristics of its host-parasite relationship were described. The survey showed that all ten investigated species of fish (representing six different families) were infected with C. irritans with similar susceptibility. In chemoattraction assays, sera and mucus collected from investigated fish strongly attracts C. irritans theronts. Sera collected from infected orange-spotted groupers and yellow spotted grunts (Plectorhynchus cinctus) could immobilize C. irritans theronts, and their immobilization titers were 1:40 and 1:6.7, respectively. The surface antigens of C. irritans were demonstrated by indirect immunofluorescence and immunostaining assays using immune orange-spotted grouper serum and a monoclonal antibody against grouper IgM.
在中国南方广东省惠州市地区，对主要海水养殖鱼类品种进行了寄生性纤毛虫刺激隐核虫宿主范围的调查，并描述了其宿主 - 寄生虫关系的一些特征。调查显示，所调查的所有十种鱼类（代表六个不同科）均感染刺激隐核虫，且易感性相似。在化学吸引试验中，从所调查鱼类收集的血清和黏液能强烈吸引刺激隐核虫幼虫。从感染的斜带石斑鱼和黄斑胡椒鲷收集的血清可使刺激隐核虫幼虫固定，其固定效价分别为1:40和1:6.7。利用免疫斜带石斑鱼血清和抗石斑鱼IgM单克隆抗体，通过间接免疫荧光和免疫染色试验证实了刺激隐核虫的表面抗原。
