Bódalo Antonio, Bastida Josefa, Máximo M Fuensanta, Montiel M Claudia, Gómez María, Murcia M Dolores
Chemical Engineering Department, University of Murcia, Campus de Espinardo, Murcia, Spain.
Bioprocess Biosyst Eng. 2008 Oct;31(6):587-93. doi: 10.1007/s00449-008-0207-7. Epub 2008 Feb 13.
Horseradish peroxidase (HRP) and soybean peroxidase (SBP) were covalently immobilized onto aldehyde glass through their amine groups. The activity yield and the protein content for the immobilized SBP were higher than for the immobilized HRP. When free and immobilized peroxidases were tested for their ability to remove 4-chlorophenol from aqueous solutions, the removal percentages were higher with immobilized HRP than with free HRP, whereas immobilized SBP needs more enzyme to reach the same conversion than free enzyme. In the present paper the two immobilized derivatives are compared. It was found that at an immobilized enzyme concentration in the reactor of 15 mg l(-1), SBP removed 5% more of 4-chlorophenol than HRP, and that a shorter treatment was necessary. Since immobilized SBP was less susceptible to inactivation than HRP and provided higher 4-chlorophenol elimination, this derivative was chosen for further inactivation studies. The protective effect of the immobilization against the enzyme inactivation by hydrogen peroxide was demonstrated.
辣根过氧化物酶（HRP）和大豆过氧化物酶（SBP）通过其氨基共价固定在醛基玻璃上。固定化SBP的活性产率和蛋白质含量高于固定化HRP。当测试游离和固定化过氧化物酶从水溶液中去除4-氯苯酚的能力时，固定化HRP的去除率高于游离HRP，而固定化SBP需要比游离酶更多的酶才能达到相同的转化率。在本文中，对两种固定化衍生物进行了比较。发现在反应器中固定化酶浓度为15 mg l(-1)时，SBP去除的4-氯苯酚比HRP多5%，并且所需的处理时间更短。由于固定化SBP比HRP更不易失活，且能提供更高的4-氯苯酚去除率，因此选择该衍生物进行进一步的失活研究。证明了固定化对过氧化氢使酶失活的保护作用。
