Guzik Urszula, Hupert-Kocurek Katarzyna, Wojcieszyńska Danuta
University of Silesia in Katowice, Faculty of Biology and Environmental Protection, Department of Biochemistry, Jagiellonska 28, 40-032 Katowice, Poland.
Molecules. 2014 Jun 27;19(7):8995-9018. doi: 10.3390/molecules19078995.
The main objective of the immobilization of enzymes is to enhance the economics of biocatalytic processes. Immobilization allows one to re-use the enzyme for an extended period of time and enables easier separation of the catalyst from the product. Additionally, immobilization improves many properties of enzymes such as performance in organic solvents, pH tolerance, heat stability or the functional stability. Increasing the structural rigidity of the protein and stabilization of multimeric enzymes which prevents dissociation-related inactivation. In the last decade, several papers about immobilization methods have been published. In our work, we present a relation between the influence of immobilization on the improvement of the properties of selected oxidoreductases and their commercial value. We also present our view on the role that different immobilization methods play in the reduction of enzyme inhibition during biotechnological processes.
酶固定化的主要目的是提高生物催化过程的经济性。固定化使酶能够长时间重复使用，并使催化剂与产物更容易分离。此外，固定化还能改善酶的许多特性，如在有机溶剂中的性能、pH耐受性、热稳定性或功能稳定性。增加蛋白质的结构刚性并稳定多聚体酶，防止与解离相关的失活。在过去十年中，已经发表了几篇关于固定化方法的论文。在我们的工作中，我们阐述了固定化对所选氧化还原酶性质改善的影响与其商业价值之间的关系。我们还阐述了不同固定化方法在生物技术过程中减少酶抑制方面所起的作用。
