Liu Qing, Puche Adam C, Wang Jia Bei
Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland, Baltimore, MD 21201, USA.
Neurochem Res. 2008 Jul;33(7):1263-76. doi: 10.1007/s11064-007-9578-4. Epub 2008 Feb 13.
Protein kinase C interactive protein (PKCI; also known as histidine triad protein, HINT1) is a small intracellular protein widely expressed in tissues from both the peripheral and CNS. Although the structure of this protein is well characterized, the functional aspect and cellular distribution of the protein remain unknown, especially in CNS. To analyze the expression pattern of PKCI/HINT1 we used antibodies against either the whole recombinant protein or a peptide epitope of PKCI/HINT1. We find widespread of PKCI/HINT1 expression in the mouse CNS by Western blot and immunostaining. Our data indicates that PKCI/HINT1 is present broadly throughout the regions of CNS with relatively high abundance in olfactory system, cerebral cortex, hippocampus and part of thalamus, hypothalamus, midbrain, pons and medulla. On the cellular level, PKCI/HINT1 immunoreactivity is primarily located in neurons and neuronal processes. This study provides the anatomical evidence for the potential roles of PKCI/HINT1 in neuronal function.
蛋白激酶C相互作用蛋白（PKCI；也称为组氨酸三联体蛋白，HINT1）是一种小的细胞内蛋白，在外周组织和中枢神经系统组织中广泛表达。尽管该蛋白的结构已得到充分表征，但其功能方面和细胞分布仍然未知，尤其是在中枢神经系统中。为了分析PKCI/HINT1的表达模式，我们使用了针对整个重组蛋白或PKCI/HINT1肽表位的抗体。通过蛋白质印迹和免疫染色，我们发现PKCI/HINT1在小鼠中枢神经系统中广泛表达。我们的数据表明，PKCI/HINT1广泛存在于中枢神经系统的各个区域，在嗅觉系统、大脑皮层、海马体以及丘脑、下丘脑、中脑、脑桥和延髓的部分区域中含量相对较高。在细胞水平上，PKCI/HINT1免疫反应性主要位于神经元和神经突中。本研究为PKCI/HINT1在神经元功能中的潜在作用提供了解剖学证据。
