De Leenheer A P, Cruyl A A
J Chromatogr Sci. 1976 Sep;14(9):434-7. doi: 10.1093/chromsci/14.9.434.
A procedure is described for the separation of Vitamin D and related compounds by gas-liquid chromatography using flame ionization detection. The method involves a two-step conversion: isomerization to the corresponding (all trans) isotachysterol(s) followed by methyl ether derivatization of the alcohol group(s). The procedure provides a means for identification as well as a possible basis for quantitation of Vitamin D and analogs.
描述了一种通过使用火焰离子化检测的气液色谱法分离维生素D及相关化合物的方法。该方法涉及两步转化：异构化为相应的（全反式）异速甾醇，然后对醇基进行甲基醚衍生化。该方法提供了一种鉴定手段以及对维生素D及其类似物进行定量的可能基础。
