来自大肠杆菌的6-丙酮酰四氢蝶呤合酶同源物的纯化、结晶及初步晶体学分析
Purification, crystallization and preliminary crystallographic analysis of a 6-pyruvoyltetrahydropterin synthase homologue from Esherichia coli.
作者信息
Seo Kyung Hye, Kim Hye Lim, Park Young Shik, Jeon Che Ok, Lee Kon Ho
机构信息
Division of Applied Life Science, Gyeongsang National University, Jinju 660-711, Republic of Korea.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Feb 1;64(Pt 2):105-7. doi: 10.1107/s1744309108000626.
Abstract
6-Pyruvoyltetrahydropterin synthase from E. coli (ePTPS) has been crystallized using the hanging-drop vapour-diffusion method. Hexagonal- and rectangular-shaped crystals were obtained. Diffraction data were collected from the hexagonal and rectangular crystals to 3.0 and 2.3 A resolution, respectively. The hexagonal plate-shaped crystals belonged to space group P321, with unit-cell parameters a = b = 112.59, c = 68.82 A , and contained two molecules in the asymmetric unit. The rectangular crystals belonged to space group I222, with unit-cell parameters a = 112.76, b = 117.66, c = 153.57 A , and contained six molecules in the asymmetric unit. The structure of ePTPS in both crystal forms has been determined by molecular replacement.
摘要
利用悬滴气相扩散法使来自大肠杆菌的6-丙酮酰四氢蝶呤合酶（ePTPS）结晶。获得了六边形和矩形晶体。分别从六边形和矩形晶体收集到分辨率为3.0 Å和2.3 Å的衍射数据。六边形板状晶体属于空间群P321，晶胞参数a = b = 112.59，c = 68.82 Å，不对称单位中含有两个分子。矩形晶体属于空间群I222，晶胞参数a = 112.76，b = 117.66，c = 153.57 Å，不对称单位中含有六个分子。两种晶体形式的ePTPS结构均已通过分子置换法确定。
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