Macon W R, Casey T T, Kinney M C, Collins R D, Cousar J B
Department of Pathology, Vanderbilt University Medical Center, Nashville, Tennessee 37232.
Am J Clin Pathol. 1991 May;95(5):696-701. doi: 10.1093/ajcp/95.5.696.
Paraffin-embedded sections of 77 peripheral T-cell lymphomas (PTCLs) were stained with several monoclonal antibodies, including the preferential T-cell markers Leu-22 (L60[CD43]) and UCHL1 (CD45RO). The staining characteristics of L60 and UCHL1 were compared to determine the value of each in the immunophenotypic analysis of PTCLs. Lineage specificity was evaluated among 39 B-cell lymphomas and 33 cases of Hodgkin's disease (HD). L60 and/or UCHL1 stained 95% of PTCLs, whereas L60 and UCHL1 alone stained 90% and 69% of cases, respectively. L60 demonstrated significantly greater numbers of immunopositive tumor cells than UCHL1 in 37% of the PTCL cases, principally because of enhanced marking of large, neoplastic cells. UCHL1 was a better marker in only 10% of the PTCL cases. L60 stained 33% of B-cell lymphomas, usually small lymphocytic or lymphoplasmacytic types. UCHL1 stained only 8% of B-cell lymphomas, all large-cell types. L60 and UCHL1 stained Reed-Sternberg cells and variants in three cases of nodular sclerosing HD. These results suggest that both L60 and UCHL1 are useful markers of PTCLs in routinely processed tissue. L60 is a more sensitive marker of large neoplastic T-cells than UCHL1 but is less lineage-specific. These antibodies are most effective when used as part of a panel of monoclonal antibodies.
对77例外周T细胞淋巴瘤（PTCL）的石蜡包埋切片用几种单克隆抗体进行染色，包括T细胞优先标志物Leu-22（L60[CD43]）和UCHL1（CD45RO）。比较L60和UCHL1的染色特征，以确定它们在PTCL免疫表型分析中的价值。在39例B细胞淋巴瘤和33例霍奇金病（HD）中评估谱系特异性。L60和/或UCHL1对95%的PTCL进行了染色，而单独使用L60和UCHL1染色的病例分别为90%和69%。在37%的PTCL病例中，L60显示免疫阳性肿瘤细胞数量明显多于UCHL1，主要是因为大型肿瘤细胞的标记增强。在仅10%的PTCL病例中，UCHL1是更好的标志物。L60对33%的B细胞淋巴瘤进行了染色，通常是小淋巴细胞或淋巴浆细胞类型。UCHL1仅对8%的B细胞淋巴瘤进行了染色，均为大细胞类型。在3例结节硬化型HD中，L60和UCHL1对里德-斯腾伯格细胞及其变异体进行了染色。这些结果表明，L60和UCHL1在常规处理的组织中都是PTCL的有用标志物。L60是比UCHL1更敏感的大型肿瘤性T细胞标志物，但谱系特异性较差。这些抗体作为单克隆抗体组合的一部分使用时最有效。
