Lee Su-Ui, Rhee Myung chull, Min Yong Ki, Kim Seong Hwan
Laboratory of Chemical Genomics, Korea Research Institute of Chemical Technology, Yuseong-gu, Daejeon 305-600, South Korea.
Cell Biol Int. 2008 Apr;32(4):401-5. doi: 10.1016/j.cellbi.2007.12.009. Epub 2008 Jan 10.
A variety of investigations on peroxiredoxins (Prxs) in different types of cancer have been carried out, but the estrogen-related function of Prxs in breast cancer has not yet been studied. In order to study the involvement of Prxs in the growth of breast cancer cells by estrogen, we evaluated the effect of mitogenic estrogen metabolites on the expression of Prx isoforms (I to VI) in MCF-7 cells and found that the transcript/protein expression of Prx IV was significantly induced by 16alpha-hydroxyestrone (OHE1) under both serum-free and serum conditions. In addition, treatment with Prx IV-specific siRNA significantly inhibited the 16alpha-OHE1-induced proliferation of MCF-7 cells. These results suggested that Prx IV involved in the 16alpha-OHE1-induced proliferation of MCF-7 cells has a proliferative effect and may be related to cancer development or progression.
针对不同类型癌症中的过氧化物还原酶（Prxs）已开展了多种研究，但Prxs在乳腺癌中与雌激素相关的功能尚未得到研究。为了研究Prxs通过雌激素参与乳腺癌细胞生长的情况，我们评估了促有丝分裂雌激素代谢物对MCF-7细胞中Prx亚型（I至VI）表达的影响，发现在无血清和有血清条件下，16α-羟基雌酮（OHE1）均可显著诱导Prx IV的转录本/蛋白表达。此外，用Prx IV特异性小干扰RNA处理可显著抑制16α-OHE1诱导的MCF-7细胞增殖。这些结果表明，参与16α-OHE1诱导的MCF-7细胞增殖的Prx IV具有增殖作用，可能与癌症发展或进展有关。
