Choi Cheol Young, An Kwang Wook, Choi Yong Ki, Jo Pil Gue, Min Byung Hwa
Division of Marine Environment and Bioscience, Korea Maritime University, Busan, Korea.
J Exp Zool A Ecol Genet Physiol. 2008 Apr 1;309(4):206-14. doi: 10.1002/jez.449.
We isolated the warm temperature acclimation-related protein 65-kDa (Wap65) cDNA from the liver of black porgy and investigated the expression by increasing water temperature in black porgy, Acanthopagrus schlegeli. Black porgy Wap65 full-length cDNA consists of 1,338 nucleotides, including an open reading frame, predicted to encode a protein of 425 amino acids and showed high homology to pufferfish (79%), Medaka (73%), carp (70%), and goldfish (68%) Wap65. Increase in water temperature (20 degrees C --> 30 degrees C; 1 degrees C/day) induced the rise of Wap65 mRNA expression in liver of black porgy. Also, the levels of cortisol and glucose in plasma were significantly higher at 30 degrees C than at 20 degrees C. To determine the high water temperature stressor specificity of the induction of Wap65, black porgy were transferred from seawater (SW) to freshwater (FW) for 24 hr. Wap65 expression was not detected when the fish were transferred from SW to FW (in fish transferred from SW to FW), although the levels of cortisol and glucose in plasma were increased. These results suggest that increase in Wap65 gene is related to high water temperature stress and play important roles in high water temperature environment of black porgy.
我们从黑鲷肝脏中分离出与暖温驯化相关的65 kDa蛋白（Wap65）的cDNA，并通过提高黑鲷（Acanthopagrus schlegeli）水温来研究其表达情况。黑鲷Wap65全长cDNA由1338个核苷酸组成，包括一个开放阅读框，预计编码一个425个氨基酸的蛋白质，与河豚（79%）、青鳉（73%）、鲤鱼（70%）和金鱼（68%）的Wap65具有高度同源性。水温升高（20℃→30℃；每天1℃）诱导黑鲷肝脏中Wap65 mRNA表达升高。此外，30℃时血浆中皮质醇和葡萄糖水平显著高于20℃时。为了确定诱导Wap65的高水温应激源特异性，将黑鲷从海水（SW）转移到淡水（FW）中24小时。当鱼从SW转移到FW时（从SW转移到FW的鱼），未检测到Wap65表达，尽管血浆中皮质醇和葡萄糖水平升高。这些结果表明，Wap65基因的增加与高水温应激有关，并且在黑鲷的高水温环境中起重要作用。
