Barakonyi Aliz, Miko Eva, Varga Peter, Szekeres-Bartho Julia
Department of Medical Microbiology and Immunology, Medical School, Pecs University, and Department of Obstetrics and Gynecology, County Hospital, Pecs, Hungary.
Am J Reprod Immunol. 2008 Mar;59(3):201-5. doi: 10.1111/j.1600-0897.2007.00555.x.
An altered function of the maternal immune system creates a favorable environment for the developing fetus during pregnancy. At term, new regulatory mechanisms are activated, to initiate labor. Earlier we showed that in peripheral blood of pregnant women gamma/delta T cells of cytotoxic phenotype are replaced by those of a non-cytotoxic phenotype. Here we studied the Vgamma and Vdelta chain usage of peripheral gamma/delta T cells from women in labor.
Vgamma and Vdelta chain expression on peripheral blood lymphocytes obtained at the 3rd trimester of pregnancy and during parturition were examined by immuncytochemistry and flow cytometry.
Increased % of Vgamma9/Vdelta2 and decreased % of Vgamma4/Vdelta1 T cells were found in peripheral blood during labor, together with unaltered percentages of single Vgamma+ or Vdelta+ cells. The initially high Vgamma4/Vdelta1 to Vgamma9/Vdelta2 ratio decreased during labor.
The initiation of labor is characterized by an altered V-chain usage of gamma/delta T cells.
孕期母体免疫系统功能的改变为发育中的胎儿创造了有利环境。足月时，新的调节机制被激活以启动分娩。此前我们发现，孕妇外周血中具有细胞毒性表型的γ/δ T细胞被非细胞毒性表型的细胞所取代。在此，我们研究了分娩期妇女外周血γ/δ T细胞的Vγ和Vδ链使用情况。
通过免疫细胞化学和流式细胞术检测妊娠晚期和分娩期间获得的外周血淋巴细胞上Vγ和Vδ链的表达。
分娩期间外周血中Vγ9/Vδ2 T细胞百分比增加，Vγ4/Vδ1 T细胞百分比降低，同时单个Vγ+或Vδ+细胞的百分比未改变。最初较高的Vγ4/Vδ1与Vγ9/Vδ2比值在分娩期间降低。
分娩的启动以γ/δ T细胞V链使用的改变为特征。
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