Vinokurov M G, Pechatnikov V A
Ukr Biokhim Zh (1978). 1991 Jan-Feb;63(1):67-71.
Ca2(+)-ATPase of sarcoplasmic reticulum was reconstituted in the proteoliposomes by the salting out procedure. Triton X-100, C12E8 and Lubrol PX were used for the solubilization of the Ca2(+)-ATPase. Using fluorescent probes (diS-C3-(5), chlortetracycline) as well pH-measuring method, the functional of the reconstituted Ca2(+)-ATPase was comparatively studied in three types of proteoliposomes. The efficiency of Ca2(+)-ATPase grew in the following detergent order: Triton X-100, C12E8, Lubrol PX.
通过盐析法将肌浆网的Ca2(+)-ATP酶重组到蛋白脂质体中。使用Triton X-100、C12E8和Lubrol PX溶解Ca2(+)-ATP酶。使用荧光探针（双硫腙-C3-(5)、金霉素）以及pH测量方法，对三种类型蛋白脂质体中重组Ca2(+)-ATP酶的功能进行了比较研究。Ca2(+)-ATP酶的效率按以下去污剂顺序增加：Triton X-100、C12E8、Lubrol PX。
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