Wang Xiusong, Liu Yadong, Lei Yanlin, Zhou Dongming, Fu Yu, Che Yi, Xu Ruchang, Yu Hualin, Hu Xintian, Ma Yuanye
Laboratory of Primate Neuroscience Research and Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, #32 Jiao Chang Dong Lu, Kunming, Yunnan, 650223, PR China.
Neurosci Lett. 2008 Mar 15;433(3):178-82. doi: 10.1016/j.neulet.2008.01.007. Epub 2008 Jan 10.
The aim of this study was to investigate the effect of extremely low-frequency electromagnetic field (ELF-EMF) exposure during morphine treatment on dopamine D2 receptor (D2R) density in the rat dorsal hippocampus following withdrawal. Rats were exposed to ELF-EMF (20 Hz, 14 mT) or sham exposed for 1h per day before injection of morphine (10mg/kg, i.p.) once daily for 12 days. The saline control group was sham exposed for the same period. Immunohistochemistry was used to detect the density of D2Rs on the 1st, 3rd and 5th morphine withdrawal days. The results showed that the density of D2Rs in sham-exposed morphine-treated rats on the 1st and 3rd days of morphine withdrawal was significantly lower than that of the saline control group. The ELF-EMF-exposed morphine group also exhibited a significantly lower density of D2Rs on the 1st and 3rd withdrawal days relative to the sham-exposed morphine group. However, the D2R density in both groups tended to recover as morphine withdrawal days increased. The results suggest that dorsal hippocampal D2Rs are sensitive to morphine withdrawal and that this is potentiated by ELF-EMF pre-exposure during morphine treatment.
本研究旨在探讨吗啡治疗期间暴露于极低频电磁场（ELF-EMF）对大鼠戒断后背侧海马中多巴胺D2受体（D2R）密度的影响。在每天注射一次吗啡（10mg/kg，腹腔注射），持续12天之前，将大鼠每天暴露于ELF-EMF（20Hz，14mT）或假暴露1小时。生理盐水对照组在同一时期进行假暴露。在吗啡戒断的第1、3和5天，使用免疫组织化学检测D2R的密度。结果显示，在吗啡戒断的第1天和第3天，假暴露的吗啡处理大鼠的D2R密度显著低于生理盐水对照组。与假暴露的吗啡组相比，ELF-EMF暴露的吗啡组在戒断的第1天和第3天也表现出显著较低的D2R密度。然而，随着吗啡戒断天数的增加，两组的D2R密度都有恢复的趋势。结果表明，背侧海马D2R对吗啡戒断敏感，并且在吗啡治疗期间ELF-EMF预暴露会增强这种敏感性。
